Recombinant Rat Krueppel-like factor 2 (Klf2)

Code CSB-YP875373RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP875373RA
MSDS
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Source E.coli
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Code CSB-EP875373RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP875373RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP875373RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Klf2
Uniprot No.
Alternative Names
Klf2; LklfKrueppel-like factor 2; Lung krueppel-like factor
Species
Rattus norvegicus (Rat)
Expression Region
1-351
Target Protein Sequence
MALSEPILPS FATFASPCER GLQERWPRNE PEAGSTDEDL NSVLDFILSM GLDGLGAENP PEPPPQPPPP AFYYPEPGAP PPYGTPAAGL GTELLRPDLD APQGPALHGR FLLAPPGRLV KAEPPEVDGG GYGCAAGLAR GPRGLKLEGA LGATGACMRG PAGRPPPPSD TPPLSPDGPP RLPAPGPRNP FPPPFGPGPS FGGPGPALHY GPPAPGAFGL FDDAAAALGL APPATRGLLT PPSSPLELLE AKPKRGRRSW PRKRAATHTC SYTNCGKTYT KSSHLKAHLR THTGEKPYHC NWDGCGWKFA RSDELTRHYR KHTGHRPFQC HLCDRAFSRS DHLALHMKRH M
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription. Might be involved in transcriptional regulation by modulating the binding of the RARA nuclear receptor to RARE DNA elements.
Gene References into Functions
  1. KLF2 was remarkably downregulated in bleomycin-treated rats, both in mRNA and protein levels. Additionally, overexpression of KLF2 attenuated the destruction of the alveolar space and pulmonary interstitial collagen hyperplasia. PMID: 29079188
  2. Transcription factor Kruppel-like factor 2 (KLF2) of the zinc finger family of DNA-binding proteins has recently emerged as a major molecular switch that controls endothelial homeostasis PMID: 27855271
  3. Cirrhotic rats treated with simvastatin showed hepatic up regulation in KLF2 protein and reduction in liver fibrosis. PMID: 25500203
  4. Upregulation of hepatic endothelial KLF2-derived transcriptional programs by statins confers vasoprotection and stellate cells deactivation. PMID: 22989565
  5. The expression of KLF2 is significantly up-regulated in chronic obstructive pulmonary disease. PMID: 22737924
  6. results provide evidence for the first time that lung kruppel like factor is down-regulated in the later stages of lung development, may impair lung development and maturation, resulting in pulmonary hypoplasia in the nitrofen CDH model. PMID: 21053160
  7. LKLF gene is not defective in diabetic prone BB rats and its expression is not compromised by mutation. (Klf2) PMID: 15033788

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Subcellular Location
Nucleus.
Protein Families
Krueppel C2H2-type zinc-finger protein family
Database Links
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