Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Mgll

Uniprot No.

Q8R431

Research Area

Cardiovascular

Alternative Names

Mgll; Mgl2; Monoglyceride lipase; MGL; EC 3.1.1.23; Monoacylglycerol lipase; MAGL

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

1-303aa

Target Protein Sequence

MPEASSPRRTPQNVPYQDLPHLVNADGQYLFCRYWKPSGTPKALIFVSHGAGEHCGRYDELAQMLKRLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDLLQHVNTVQKDYPEVPVFLLGHSMGGAISILAAAERPTHFSGMILISPLILANPESASTLKVLAAKLLNFVLPNISLGRIDSSVLSRNKSEVDLYNSDPLICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSPSQDKTLKMYEGAYHVLHKELPEVTNSVLHEINTWVSHRIAVAGARCLP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

34.8 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To achieve the expression of the recombinant Rat Mgll protein in Yeast cells, a DNA fragment encoding the Rat Mgll protein (1-303aa) is inserted into a plasmid vector, which is then transferred to Yeast cells. After screening positive cells, they are cultured and induced to generate the Mgll protein. A N-terminal 6xHis tag is attached to the protein. Cells are lysed to collect the recombinant Rat Mgll protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the obtained recombinant Rat Mgll protein is over 90%.

Target Background

Function

Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.

Gene References into Functions

4 weeks of post-weaning social isolation increased aggressive grooming of a novel same-sex conspecific in male and female rats. Treatment with the MAGL inhibitor MJN110 eliminated this increase in aggressive grooming in both males and females, without reducing non-aggressive social behavior. Activation of the medial prefrontal cortex was increased by social interaction but was blunted by MJN110 only in females. PMID: 29292159
MGL has an important role in insulin secretion PMID: 26867016
expression of MAGL is distributed in certain cell types and time-dependently expressed in skeletal muscles after trauma, suggesting that MAGL may be involved in inflammatory response, fibrogenesis and regeneration during skeletal muscle wound healing PMID: 25921063
MAGL inhibitor JZL184 is able to inhibit the inflammatory response and interfere with contused muscle healing. PMID: 25912803
results suggest that MAGL regulates phagocytosis, but not LPS-mediated cytokine induction in microglia PMID: 26166819
the current findings support the therapeutic potential of FAAH and monoacylglycerol lipase inhibitors as a novel pharmacotherapy for pain PMID: 25041240
Data indicate that nerve growth factor (NGF) controls monoacylglycerol lipase (MGL) degradation in vitro and in vivo. PMID: 23319656
Pretreatment with MAGL inhibitor URB602 significantly reduces neonatal hypoxic-ischemic brain damage and improves functional outcome. PMID: 22821058
Maternal deprivation induced a significant decrease in MAGL immunoreactivity in the rat hippocampal CA3 and CA1 areas, more marked in males than in females. PMID: 20599824
hydrolysis by means of monoglyceride lipase is a primary mechanism for 2-arachidonoylglycerol inactivation in intact neurons PMID: 12136125
MGL is localized in the enteric nervous system where endocannabinoids regulate intestinal motility. MGL is highly expressed in the epithelium, where this enzyme may have digestive or other functions yet to be determined. PMID: 18948437

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Subcellular Location

Cytoplasm, cytosol. Membrane; Peripheral membrane protein.

Protein Families

AB hydrolase superfamily, Monoacylglycerol lipase family

Tissue Specificity

Ubiquitous. Highly expressed in adipose tissue, adrenal gland, ovary, heart, spleen, lung, skeletal muscle, kidney and testis. Highly expressed throughout the brain.

Database Links

KEGG: rno:29254

STRING: 10116.ENSRNOP00000030271

UniGene: Rn.40396

Code	CSB-YP013787RA
Abbreviation	Recombinant Rat Mgll protein
MSDS	MSDS Datasheet
Size	$368
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Rat Monoglyceride lipase (Mgll)

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