Recombinant Rat Myosin-10 (Myh10), partial

Code CSB-YP888354RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP888354RA
MSDS
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Source E.coli
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Code CSB-EP888354RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP888354RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP888354RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Myh10
Uniprot No.
Alternative Names
Myh10; Myosin-10; Cellular myosin heavy chain; type B; Myosin heavy chain 10; Myosin heavy chain; non-muscle IIb; Non-muscle myosin heavy chain B; NMMHC-B; Non-muscle myosin heavy chain IIb; NMMHC II-b; NMMHC-IIB
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9. Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.
Gene References into Functions
  1. Study suggests that NMIIB remains constitutively active in support of methamphetamine associations, continuing to drive actin polymerization, such that memories associated with methamphetamine can be selectively targeted without the need for retrieval days to weeks after learning PMID: 26239291
  2. NM II maintains proper morphology of neuronal growth cones and the distal axon by regulating actin dynamics through the guanine nucleotide exchange factor-Rho GTPase signaling pathway. PMID: 24752242
  3. NM II manages several major processes involved in the beta cell response to glucose, including gamma-actin protrusion and remodelling, FA assembly and turnover, and the secretory granule approach to the basal membrane PMID: 23354122
  4. IIb MHC transcriptional regulation during muscle maturation to the adult state is consistent with the previously defined model of antisense-mediated coordination of genomically adjacent MHC genes, as is evident in the transcriptionally divergent euthyroid and hypothyroid states. PMID: 22262309
  5. These data support the idea that Myosin II motor-force generation is a general mechanism that supports memory consolidation in the mammalian CNS. PMID: 22174310
  6. myosin IIb motor activity is downstream of LTP induction and is necessary for the emergence of actin structures that stabilize an early phase of LTP, and myosin IIb activity contributes to an actin-dependent process that underlies memory consolidation PMID: 20797537
  7. These results suggest that the mesangial expression of SMemb is related to glomerular matrix accumulation and that angiotensin II mediates both mesangial processes via AT1 receptors. PMID: 11961408
  8. non-muscle myosin II and myosin light chain kinase are downstream targets for vasopressin signaling in the renal collecting duct PMID: 15347643
  9. the SMB isoform is the predominant form expressed in rat veins, providing one possible mechanism for the faster response of veins to vasoconstrictors PMID: 17377397
  10. Isolated Myh10 from rat skeletal muscle and report amino sequence. PMID: 17973598
  11. These results provide important insights related to how proximal IIb MHC promoter elements regulate the increased expression of the IIb MHC gene in response to inactivity of a predominantly slow postural muscle. PMID: 19623632

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Subcellular Location
Cell projection, lamellipodium.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Database Links
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