Product Details

Purity

>85% (SDS-PAGE)

Target Names

Nol3

Uniprot No.

Q62881

Alternative Names

Nol3; ArcNucleolar protein 3; Apoptosis repressor with CARD

Species

Rattus norvegicus (Rat)

Expression Region

1-221

Target Protein Sequence

MGNMQERPSE TIDRERKRLV ETLQADSGLL LDALVARGVL TGPEYEALDA LPDAERRVRR LLLLVQSKGE AACQELLRCA QQTVSMPDPA WDWQHVGPGY RDRSYDPPCP GHWTPEAPSS GTTCPGLPRA SEEEEIGGPE DSEAVQPRTP EEPELEAEAT KGDEPDLEQE MEPEPEPEVE PEPEPEPEPE PEPEPEPEPE PEPEREPDFQ EGDESEGCEN T

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting the amount of soluble CASP8 available for DISC-mediated activation. Inhibits intrinsic apoptotic pathway in response to a wide range of stresses, through its interaction with BAX resulting in BAX inactivation, preventing mitochondrial dysfunction and release of pro-apoptotic factors. Inhibits calcium-mediated cell death by functioning as a cytosolic calcium buffer, dissociating its interaction with CASP8 and maintaining calcium homeostasis. Negatively regulates oxidative stress-induced apoptosis by phosphorylation-dependent suppression of the mitochondria-mediated intrinsic pathway, by blocking CASP2 activation and BAX translocation. Negatively regulates hypoxia-induced apoptosis in part by inhibiting the release of cytochrome c from mitochondria in a caspase-independent manner. Also inhibits TNF-induced necrosis by preventing TNF-signaling pathway through TNFRSF1A interaction abrogating the recruitment of RIPK1 to complex I. Finally through its role as apoptosis repressor, promotes vascular remodeling through inhibition of apoptosis and stimulation of proliferation, in response to hypoxia. Inhibits too myoblast differentiation through caspase inhibition.

Gene References into Functions

The results of the present study revealed that ARC inhibition promotes the apoptosis of pulmonary arterial smooth muscle cells. PMID: 28731195
Foxo3a maintains calcium homeostasis and inhibits cardiac apoptosis through trans-activation of the ARC promoter. PMID: 23382383
Data show that low-dose mineralocorticoid receptor antagonists reduce infarct size and apoptosis in the reperfused myocardium by preventing the apoptosis repressor with caspase recruitment domain (ARC) degradation. PMID: 22508833
A novel cardiac pathway is identified in which anesthetic preconditioning results in increased phosphorylation of nucleolar protein 3 following inhibition of calcineurin increase. PMID: 21233493
ARC protein is expressed in vascular smooth muscle and is significantly reduced in several target tissues during hypertension. PMID: 20026055
Down-regulation of ARC contributes to vulnerability of hippocampal neurons to ischemia/hypoxia PMID: 12753927
mitochondrial ARC levels increased with age and were lower in calorie-restricted rats than in age-matched controls PMID: 14732288
ARC can protect against cell death by interfering with activation of the mitochondrial death pathway through the interaction with Bax, preventing mitochondrial dysfunction and release of pro-apoptotic factors. PMID: 15004034
Unexpectedly, ARC was localized almost exclusively to the nuclei of cancer cells, which was unlike the cytoplasmic localization of ARC in non-cancer cells PMID: 15848180
ARC-overexpression inhibited myoblast differentiation associated caspase-3 activation, suggesting ARC inhibits myogenic differentiation through caspase inhibition. PMID: 17292893
preconditioning-induced suppression of the release of mitochondrial cytochrome c to cytosol and caspase-3 activation could be abolished by the inhibition of endogenous ARC expression using ARC antisense oligonucleotides. PMID: 17594520
reveal a novel p53 apoptotic pathway in which it initiates apoptosis by transcriptionally repressing ARC PMID: 17998337
catalase, CK2, and ARC constitute an anti-hypertrophic pathway in the heart. PMID: 18171680
Novel cardiac apoptotic pathway in which ARC is dephosphorylated by calcineurin, which could be a component in the cardiac apoptotic machinery. PMID: 19001025
ARC plays an essential role in mediating the cardioprotective effect of postconditioning against apoptosis initiated by the mitochondrial pathway. PMID: 19130235
Studies provide evidence for the protective role of anti-apoptotic ARC in Dox-induced cardiotoxicity, which makes this molecule an interesting target for future therapies. PMID: 19139834
Down-regulation of ARC (apoptosis repressor with caspase recruitment domain) contributes to vulnerability of hippocampal neurons to ischemia/hypoxia. PMID: 12753927

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Subcellular Location

Cytoplasm. Mitochondrion. Sarcoplasmic reticulum. Membrane; Lipid-anchor.

Tissue Specificity

Highly expressed in skeletal muscle, heart and medulla.

Database Links

KEGG: rno:85383

UniGene: Rn.86956

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Recombinant Rat Nucleolar protein 3 (Nol3)

Product Details

Customer Reviews and Q&A

Target Background

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