Recombinant Rat PDZ and LIM domain protein 5 (Pdlim5)

Code CSB-YP726819RA
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP726819RA
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP726819RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP726819RA
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP726819RA
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
≥85% (SDS-PAGE)
Target Names
Pdlim5
Uniprot No.
Alternative Names
Pdlim5; Enh; PDZ and LIM domain protein 5; Enigma homolog; Enigma-like PDZ and LIM domains protein
Species
Rattus norvegicus (Rat)
Expression Region
2-591
Target Protein Sequence
SNYNVSLVG PAPWGFRLQG GKDFNMPLTI SSLKDGGKAS QAHVRIGDVV LSIDGISAQG MTHLEAQNKI KACTGSLNMT LQRASAAAKS EPVAVQKGEP KEVVKPVPIT SPAVSKVTST TNMAYNKVPR PFGSVSSPKV TSIPSPSSAF TPAHAATSSH ASPPPVAAVT PPPLSASGLH ASANPSAAQC SSPPNTGKPA VHVPRQPTVT SVCSESAQEL AEGQRRGSQG DIKQQNGPPR KHIVERNTEF YHIPTHSDAS KKRLIEDTED WRPRTGTTQS RSFRILAQIT GTEHLKESEN DNAKKANSTP EPSQQSASPL SAAESLESPG SNRPVVAGLR SAAAFKPVGS TSVKSPSWQR PNQAAPSTGR ISNSASSSGT GAPMKPAVGP PQPSDQDTLV QRAEHIPAGK RTPMCAHCNQ AIRGPFLVAL GKSWHPEEFN CAHCKNTMAY IGFVEEKGAL YCELCYEKFF APECGRCQRK ILGEVINALK QTWHVSCFVC VACGKPIRNN VFHLEDGEPY CETDYYALFG TICRGCEFPI EAGDMFLEAL GSTWHDTCFV CSVCCESLEG QTFFSKKDKP LCKKHAHSVN F
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Isoforms lacking the LIM domains may negatively modulate the scaffolding activity of isoform 1. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May be required to restrain postsynaptic growth of excitatory synapses. Isoform 1, but not isoform 2, expression favors spine thinning and elongation.
Gene References into Functions
  1. results suggest that, in healthy conditions, RBM20 and RBM24 cooperate to promote the expression of short ENH isoforms. PMID: 27289039
  2. ENH1 plays an essential role in CREB's activation and dependent transcription in cardiomyocytes. PMID: 26365342
  3. PDLIM5 acts as a scaffold protein by mediated PKCepsilon translocated to the membrane in PMA-induced growth cone collapse. PMID: 25524223
  4. coordinated and stepwise splicing transitions result in the production of specific ENH transcripts in mature striated muscles. PMID: 22497889
  5. Neuroligin function was critically dependent on the cytoplasmic tail, but required non-PDZ ligand. PMID: 21532576
  6. Antagonistic splice variants of enigma may play a central role in the adaptive changes of the link between mechanical stress-sensing and signalling occurring during embryonic development and/or heart hypertrophy. PMID: 20097676
  7. ENH protein, by interacting specifically with both protein kinase C (PKC)epsilon and the N-type Ca2+ channel, targets a specific PKC to its substrate to form a functional signaling complex. PMID: 12665800
  8. ENH is a restraining factor of the oncogenic activity of inhibitor of DNA binding 2 proteins in neural tumors PMID: 16549780
  9. Propose a model where ENH1 scaffolds PKD1 to Cacna1c in order to form a signalling complex that regulates the activity of cardiac L-type voltage-gated Ca(2+) channels. PMID: 18296710

Show More

Hide All

Subcellular Location
[Isoform 1]: Cell junction, synapse, postsynaptic density. Cell junction, synapse, presynapse. Cell junction, synapse, postsynapse. Cytoplasm, cytosol.; [Isoform 2]: Cell junction, synapse, postsynaptic density. Cell junction, synapse, presynapse. Cell junction, synapse, postsynapse. Cytoplasm, cytosol.
Tissue Specificity
Detected in brain, in neurons, including in hippocampal neurons, and glial cells (at protein level). Detected in heart and skeletal muscle.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2026 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1