Recombinant Rat Prostacyclin synthase (Ptgis), partial,Yeast

Code CSB-YP717293RA
MSDS
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ptgis
Uniprot No.
Alternative Names
Ptgis; Cyp8; Prostacyclin synthase; EC 5.3.99.4; Prostaglandin I2 synthase
Species
Rattus norvegicus (Rat)
Source
Yeast
Protein Length
partial
Tag Info
N-terminal His-tagged
Tag-Free
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE).
Gene References into Functions
  1. Data show that renal reparation of the acute kidney injury due to choline deficiency in some rats is not related with differences in VNTR in the promoter region of the prostacyclin synthase gene. PMID: 20925195
  2. PGIS overexpression apparently protects insulin-producing cells against cytokine toxicity via suppression of endoplasmic reticulum and mitochondrial stress-mediated cell death pathways PMID: 20159982
  3. Repeated gene transfer of PGIS into skeletal muscle attenuates monocrotaline-induced pulmonary hypertension and prolongs survival. PMID: 15684702
  4. Prostacyclin synthase protects the brain by enhancing PGI2 synthesis and creating a favorable PGI2/TXA2 ratio PMID: 16094316
  5. Overall, this study demonstrated that engagement of TLR4 with LPS induces production of PGI2 via Akt and generates VEGF through IP receptor. PMID: 17825254

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Subcellular Location
Endoplasmic reticulum membrane; Single-pass membrane protein.
Protein Families
Cytochrome P450 family
Database Links
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