Recombinant Rat RAS guanyl-releasing protein 4 (Rasgrp4)

Code CSB-YP848140RA
MSDS
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Source Yeast
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Code CSB-EP848140RA
MSDS
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Source E.coli
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Code CSB-EP848140RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP848140RA
MSDS
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Source Baculovirus
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Code CSB-MP848140RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rasgrp4
Uniprot No.
Alternative Names
Rasgrp4RAS guanyl-releasing protein 4
Species
Rattus norvegicus (Rat)
Expression Region
1-678
Target Protein Sequence
MNRKDIKRKS HQECSGKAGG HGRPRQARRH KTCPTPREIS KVMASLNLGV LSESSCSVDE LLEECIRCFD SAGSLHRGDH ILKMVLTMHS WVLPSSDLAA RLLTSYQKAA KDARELRQLQ ICYLVRYWLT HHPEVVHQEP QLEAIINRFW TTVAQEGNMA QRSLGDASNL LSPGGPGPPP LMSSPGLGKK RKVSLLFDHL ETEELAQHLT YLEFRSFQAI TPQDLRGYVL QGSVRGCPAL EGSVGLSNSV SRWVQVMVLS RPGPAQRAQV LDKFIRVAQR LRQMQNFNTL MAVTGGLCHS AISRLKDSHV HLSPDSTKAL LELTELLSSH NNYAYYRRTW ASCTDFRLPV LGVHLKDLVS LYEAHPDRLP DGRLHLPKLN SLYLRLQELA ALQRQHPPCS ANEDLLHLLT LSLDLFYTED EIYELSYARE PRCPKSLPLS PFKAPVVVEW AHGVTPKPDS ATLGQHVEQL VESVFKNYDP EGHGSISLED FEKLSANFPF ACHGLHPPPR HGSGSFSREE LTKYLLHASA ICSKLGLAFL HAFQEVTFRK PTFCHSCNGF VSTGPLWGVT KRGYRCQDCG LCCHRHCRDQ VRVECKKRPE TKGDPGPPGA PGPATPLPPT GCGSEEGLSY TLSPHLESGC HLHHAWTQTE SSHSSWEPEM VPCPAPVLPS KASSKSSV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.
Gene References into Functions
  1. RasGRP-4 is aberrantly expressed in fibroblast-like synoviocytes and helps regulate their growth. PMID: 25330932
  2. Cloning of Ras guanine nucleotide releasing protein 4, and evaluation of its expression in mast cells and their bone marrow progenitors. PMID: 12217396
  3. RasGRP4 regulates the expression of prostaglandin D2 in mast cell PMID: 12493770
Subcellular Location
Cytoplasm. Cell membrane.
Protein Families
RASGRP family
Tissue Specificity
Expressed by mast cells and their progenitors (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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