Recombinant Rat Rab proteins geranylgeranyltransferase component A 1 (Chm)

Code CSB-YP005356RA
MSDS
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Source Yeast
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Code CSB-EP005356RA
MSDS
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Source E.coli
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Code CSB-EP005356RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005356RA
MSDS
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Source Baculovirus
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Code CSB-MP005356RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Chm
Uniprot No.
Alternative Names
Chm; Rep1; Rab proteins geranylgeranyltransferase component A 1; Choroideremia protein homolog; Rab escort protein 1; REP-1
Species
Rattus norvegicus (Rat)
Expression Region
1-650
Target Protein Sequence
MADNLPSDFD VIVIGTGLPE SIIAAACSRS GQRVLHVDSR SYYGGNWASF SFSGLLSWLK EYQENNDVVT ENSMWQEQIL ENEEAIPLSS KDKTIQHVEV FCYASQDLHK DVEEAGALQK NHASVTSAQS AEAAEAAETS CLPTAVEPLS MGSCEIPAEQ SQCPGPESSP EVNDAEATGK KENSDAKSST EEPSENVPKV QDNTETPKKN RITYSQIIKE GRRFNIDLVS QLLYSRGLLI DLLIKSNVSR YAEFKNITRI LAFREGTVEQ VPCSRADVFN SKQLTMVEKR MLMKFLTFCV EYEEHPDEYR AYEGTTFSEY LKTQKLTPNL QYFVLHSIAM TSETTSCTVD GLKATKKFLQ CLGRYGNTPF LFPLYGQGEL PQCFCRMCAV FGGIYCLRHS VQCLVVDKES RKCKAVIDQF GQRIISKHFI IEDSYLSENT CSRVQYRQIS RAVLITDGSV LKTDADQQVS ILAVPAEEPG SFGVRVIELC SSTMTCMKGT YLVHLTCMSS KTAREDLERV VQKLFTPYTE IEAENEQVEK PRLLWALYFN MRDSSDISRD CYNDLPSNVY VCSGPDSGLG NDNAVKQAET LFQQICPNED FCPAPPNPED IVLDGDSSQQ EVPESSVTPE TNSETPKEST VLGNPEEPSE
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Substrate-binding subunit of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B composed of RABGGTA and RABGGTB, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Besides, a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation.
Gene References into Functions
  1. RabGGTase has evolved from a GGTase-I-like molecule that 'learned' to interact with a recycling factor (GDI) that, in turn, eventually gave rise to REP1. PMID: 18756270
Subcellular Location
Cytoplasm, cytosol.
Protein Families
Rab GDI family
Tissue Specificity
Most abundant in the heart, brain, and spleen. Lower levels seen in the lung, liver, muscle and kidney. Extremely low levels seen in the testis.
Database Links
CUSABIO guaranteed quality
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