Recombinant Rat Rho guanine nucleotide exchange factor 6 (Arhgef6)

Code CSB-YP730408RA
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Source Yeast
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Code CSB-EP730408RA
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Source E.coli
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Code CSB-EP730408RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP730408RA
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Source Baculovirus
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Code CSB-MP730408RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Arhgef6
Uniprot No.
Alternative Names
Arhgef6; Rho guanine nucleotide exchange factor 6; Rac/Cdc42 guanine nucleotide exchange factor 6
Species
Rattus norvegicus (Rat)
Expression Region
1-772
Target Protein Sequence
MNPEERVVTW LISLGVLESP KKTICDPEEF LKSSLKNGVV LCKLISRLLP GSVEKYCQEP QTEADCIDNI NDFLKGCATL QVEVFEPDDL YSGANFSKVL NTLLAVNKAT EDQLSERPCG RSSSLSATTS SQTNPQAAVP STTPEQQSEE KAAEMTENGS HQLIVKARFN FKQTNEDELS VCKGDIIYVT RVEEGGWWEG TLNGRTGWFP SNYVREIKPS ERPLSPKAVK GFDTAPLTKN YYTVVLQNIL DTEKEYAKEL QSLLVTYLRP LQSNNNLSTV EFTCLLGNFE EVCTFQQTLC QALEECSKSP ENQHKVGGCL LNLMPHFKSM YLAYCANHPS AVNVLTQHSD DLERFMENQG ASSPGILILT TSLSKPFMRL EKYVTLLQEL ERHMEDTHPD HQDILKAIIA FKSLMGQCQD LRKRKQLELQ ILSEPIQAWE GDDIKTLGNV IFMSQVVMQH GACEEKEERY FLLFSSVLIM LSASPRMSGF MYQGKVPIAG MVVTRLDEIE GNDCTFEITG STVERIVVHC NNNQDFQEWM EQLNRLTKGP ASCSSLSKTS SSSCSTHSSF SSTGQPRGPL EPPQIIKPWS LSCLRPAPPL RPSAALGYKE RMSYILKESS KSPKTMKKFL HKRKTERKTS EEEYVIRKST AALEEDAQIL KVIEAYCTSA SFQQGTRKDS VPQVLLPEEE KLIIEETRSN GQTIIEEKSL VDTVYALKDE VKELKQENKK MKQCLEEELK SRKDLEKLVR KLLKQTDESI RAESSSKTSI LQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as a RAC1 guanine nucleotide exchange factor (GEF).
Gene References into Functions
  1. c-Cbl negatively regulates alphaPix-mediated cell migration and invasion; the lack of c-Cbl in C6 and A172 glioma cells is responsible for their malignant behavior PMID: 25450678
  2. alphaPIX and GIT2 are required for neuronal differentiation. PMID: 22554054
Subcellular Location
Cell projection, lamellipodium.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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