Recombinant Rat SH3KBP1-binding protein 1 (Shkbp1)

Code CSB-YP021271RA
MSDS
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Source Yeast
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Code CSB-EP021271RA
MSDS
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Source E.coli
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Code CSB-EP021271RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021271RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP021271RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Shkbp1
Uniprot No.
Alternative Names
Shkbp1; Sb1; SH3KBP1-binding protein 1; SETA-binding protein 1
Species
Rattus norvegicus (Rat)
Expression Region
2-704
Target Protein Sequence
AVPTTAVEG VRSRGAPGEV IHLNVGGKRF STSRQTLTWI PDSFFSSLLS GRISTLKDET GAIFIDRDPT VFAPILNFLR TKELDPRGVH GSSLLHEAQF YGLTPLVRRL QVREELDRSS CGNVLFNGYL PPPVFPVKRR NRHSLVGPQQ IGGRPAPVRR SNTMPPNLGN AGLLGRMLDD RAPPSPSGQP EEPGMVRLVC GHHNWIAVAY THFLVCYRLK EASGWQLAFS SPRLDWPIER LALTARVLGG APGEHDKMVA AATGSEILLW ALQAQGGGSE IGVFHLGVPV EALFFVGNQL IATSHTGRIG VWNAVTKHWQ VQEVQPITSY DAAGSFLLLG CSNGSIYYVD VQKFPLRMKD NDLLVSELYR DPAEDGVTAL SVYLTPKTSD SGNWIEIAYG TSSGVVRVIV QHPETVGSGP QLFQTFSVHR SPVTKIMLSE KHLISVCADN NHVRTWSVTR FRGMISTQPG STPLASFKIL ALESADGLGG CSAGNDIGPY GERDDQQVFI QKVVPNASQL FVRLSSTGQR VCSVRSVDGS ATTAFTVLEC EGSRRLGSRP RRYLLTGQAN GSLAMWDLTT AMDGLGQTPA GGLTEEELMD QLEQCELSPL TSSRASFPSP SPRTSLTSLH SASSNTSLCG HRGSPSPPQA GARSRGAGSF VDRFKELARG APELRGPPTP APRPSTSLGN PLILPKNTLN ETSF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.
Subcellular Location
Lysosome.
Protein Families
KCTD3 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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