Recombinant Rat Serine protease inhibitor A3K (Serpina3k)

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Code CSB-EP361549RA
Size $388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Serpina3k
Uniprot No.
Research Area
Others
Alternative Names
Serpina3k; Spin2b; Serine protease inhibitor A3K; Serpin A3K; CPI-21; Contrapsin-like protease inhibitor 1; GHR-P63; Growth hormone-regulated proteinase inhibitor; Kallikrein-binding protein; KBP; SPI-2.3; Serine protease inhibitor 2; SPI-2; Thyroid hormone-regulated protein
Species
Rattus norvegicus (Rat)
Source
E.coli
Expression Region
21-416aa
Target Protein Sequence
DGILGRDTLPHEDQGKGRQLHSLTLASINTDFTLSLYKKLALRNPDKNVVFSPLSISAALAILSLGAKDSTMEEILEVLKFNLTEITEEEIHQGFGHLLQRLSQPEDQAEINTGSALFIDKEQPILSEFQEKTRALYQAEAFVADFKQCNEAKKFINDYVSNQTQGKIAELFSELDERTSMVLVNYLLFKGKWKVPFNPNDTFESEFYLDEKRSVKVPMMKIKDLTTPYIRDEELSCSVLELKYTGNASALFILPDQGKMQQVESSLQPETLKKWKDSLRPRIISELRMPKFSISTDYNLEEVLPELGIRKIFSQQADLSRITGTKNLHVSQVVHKAVLDVDETGTEGAAATAVTAALKSLPQTIPLLNFNRPFMLVITDNNGQSVFFMGKVTNPM
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
52.0 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The process for recombinant Rat Serpina3k protein synthesis in e.coli cells includes recombining the DNA fragment that encodes the Rat Serpina3k protein (21-416aa) into expression vector, transforming the recombinant vector into e.coli cells, screening positive cells, cultivating and inducing cells, conducting cell lysis, and performing expression analysis. The resulting recombinant Rat Serpina3k protein is purified from the cell lysate using affinity purification, with its purity exceeding 85% as determined by SDS-PAGE.

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Target Background

Function
Binds to and inhibits kallikreins. Inhibits trypsin but not chymotrypsin or elastase.
Gene References into Functions
  1. KBP suppressed lipopolysaccharide-induced TNF-alpha production via upregulating SOCS3 expression. PMID: 23129128
  2. KBP competed with (125)I-VEGF for binding to endothelial cells and down-regulated VEGF production in endothelial cells and in the retina of the oxygen-induced retinopathy rat model PMID: 12743698
  3. in a rat model of chronic mycardial infarction (MI) kallistatin improves cardiac performance and prevents post-MI-induced cardiac hypertrophy and fibrosis through its antioxidant action. PMID: 18762777
  4. An inverse relationship exists between oxidative stress and kallistatin levels in the circulation and blood vessels. Kallistatin expression is negatively regulated by oxidative stress via JNK-dependent FOXO1 activation in cultured endothelial cells. PMID: 20081110
  5. Pivotal role for alpha1-antichymotrypsin in skin repair PMID: 21693707
  6. SERPINA3K is an antifibrogenic factor, and its antifibrogenic activity is through blocking the Wnt pathway. Decreased SERPINA3K levels may contribute to the fibrosis in diabetic retinopathy. PMID: 20299474
  7. SERPINA3K is a high-affinity, endogenous antagonist of LRP6 PMID: 20351274

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Subcellular Location
Secreted.
Protein Families
Serpin family
Tissue Specificity
Liver and plasma.
Database Links
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