Product Details

Purity

>85% (SDS-PAGE)

Target Names

Slc8a3

Uniprot No.

P70549

Alternative Names

Slc8a3; Ncx3; Sodium/calcium exchanger 3; Na(+)/Ca(2+)-exchange protein 3; Solute carrier family 8 member 3

Species

Rattus norvegicus (Rat)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells, both in muscle and in brain. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A3 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline. Contributes to Ca(2+) transport during excitation-contraction coupling in muscle. In neurons, contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory. Required for normal oligodendrocyte differentiation and for normal myelination. Mediates Ca(2+) efflux from mitochondria and contributes to mitochondrial Ca(2+) ion homeostasis.

Gene References into Functions

This cardioprotective effect was potentially achieved via up-regulating p-AKT and SLC8A3. PMID: 29763795
There is a significant reduction in NCX3 expression in rats with febrile convulsions. PMID: 26608990
This study demonstrated that the change in NCX3 in the cell body was associated with a decrease in NCX3 protein in the ganglia, an increase in the peripheral nerve (sciatic) yet no change in the central root. PMID: 26041911
This study showed that normobaric hyperoxia changes the patterns of NCXs expression in the brain; up-regulates the expression of NCX3 in the in the spinal cord cord, penumbra and subcortex. PMID: 23403180
NCX3 in reverse mode was found to be another influx route for increasing [Ca2+]i, which might be involved in interstitial cells of Cajal-like cell excitability. PMID: 23688374
High levels of NCX3 overexpression increase neuronal survival following hypoxia-ischemia and improve neuronal calcium management. PMID: 22561287
calcium signaling mediated by NCX3 has a crucial role in oligodendrocyte maturation and myelin formation. PMID: 21959935
NCX3/Slc8a3, a Na(+)/Ca(2+) membrane exchanger, is highly expressed in astrocytes, both in vitro and in vivo, in response to glutamate-induced excitotoxicity. PMID: 20928830
NCX3 silencing, induced by intracerebroventricular infusion of small interfering RNA (siRNA), partially reverted postconditioning-induced neuroprotection PMID: 20628398
Brain ischemia regulates gene expression levels of Na+/Ca2+ exchanger isoform NCX3, especially in cerebral cortex. PMID: 12377375
NCX3 mRNAs were detected in the hippocampus, thalamus, amygdala, and cerebellum. The NCX3 protein was found in the specific subregions of the hippocampus, the ventral striatum, and in the cerebellar molecular layer. PMID: 12722103
Proteolytic inactivation of NCX-driven neuronal Ca2+ extrusion is responsible for the delayed excitotoxic Ca2+ deregulation and neuronal death. PMID: 15680332
Expression is depressed in heart failure. PMID: 15681692
Slc8a3 constitutes a novel additional target for the prosurvival action of Akt/protein kinase B pathway. PMID: 18079274
NCX3 protein is proposed as a distress signal able to modulate intraneuronal homeostasis of sodium and divalent calcium ions whenever neurons undergo severe anoxia. PMID: 18234895

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Perikaryon. Cell projection, dendrite. Cell projection, dendritic spine. Cell membrane, sarcolemma. Cytoplasm, sarcoplasm. Cell junction. Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasm, perinuclear region.

Protein Families

Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family, SLC8 subfamily

Tissue Specificity

Detected in neurons in brain cortex and hippocampus. Detected in pyramidal cell bodies and processes, in granule cells and interneurons in the CA1 and CA3 region of the hippocampus. Detected on astrocyte processes in brain cortex. Detected on endothelial

Database Links

KEGG: rno:140448

STRING: 10116.ENSRNOP00000041987

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Recombinant Rat Sodium/calcium exchanger 3 (Slc8a3), partial

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