Recombinant Rat THO complex subunit 5 homolog (Thoc5)

Code CSB-YP721228RA
MSDS
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Source Yeast
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Code CSB-EP721228RA
MSDS
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Source E.coli
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Code CSB-EP721228RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP721228RA
MSDS
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Source Baculovirus
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Code CSB-MP721228RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Thoc5
Uniprot No.
Alternative Names
Thoc5; Fmip; THO complex subunit 5 homolog; Fms-interacting protein
Species
Rattus norvegicus (Rat)
Expression Region
2-682
Target Protein Sequence
SSESSKKRK PKVIRSDGTP TEGKRNRSDT EQEGRYYSEE AEVDLRDPGR DYELYKYTCQ ELQRLMAEIQ DLKSKGSKDV AIEIEERRIQ SCVHFMTLKK LNRLAHIRLK KGRDQTHEAK QKVDAYHLQL QNLLYEVMHL QKEITKCLEF KSKHEEIDLV SLEEFYSEAP PNISKAEITM GDPHQQTLAR LDWELEQRKR LAEKYRECLS NKEKILKEIE VKKEYLSSLQ PRLNSIMQAS LPVQEYLFMP FDQAHKQYET ARHLPPPLYV LFVQATAYGQ ACDKTLSVAI EGSVDEAKAL FKPPEDSQDD ESDSDAEEEQ TTRRRPTLGV QLDDKRKEML KRHPLSVLLD LKCKDNSVLH LTFFYLMNLN IMTVKAKVTT AMELITPISA GDLLSPDSVL SCLYPGDHGK KTPNPANQYQ FDKVGILTLR DYVLELGHPY LWVQKLGGLH FPKEQPQHTV MADHSQSASH METTMKLLKT RVQSRLALHK QFASLEHGIV PVTSECQYLF PAKVVSRLVK WMIMAHEDYM ELHFTKDIVE AGLAGDTNLY YLALIERGTA KLQAAVVLNP GYSSIPPVFR LCLNWKGEKT NSNDDNIRAM ESEVNVCYRE LCGPRPSHQL LTNQLQRLCV LLDVYLETES HDDSVEGPKE FPQEKMCLRL FRGPSRMKPF KYNHPQGFFS HR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability. Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes.; Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
THOC5 family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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