Recombinant Rat Translation initiation factor eIF-2B subunit delta (Eif2b4)

Code CSB-YP723561RA
MSDS
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Source Yeast
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Code CSB-EP723561RA
MSDS
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Source E.coli
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Code CSB-EP723561RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP723561RA
MSDS
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Source Baculovirus
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Code CSB-MP723561RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Eif2b4
Uniprot No.
Alternative Names
Eif2b4; Eif2bd; Translation initiation factor eIF-2B subunit delta; eIF-2B GDP-GTP exchange factor subunit delta
Species
Rattus norvegicus (Rat)
Expression Region
2-524
Target Protein Sequence
AAVAVAVRE ESRSEMKTEL SPRPGAAGRE LTQEEKLQLR KEKKQQKKKR KEEKGADQEI GSAVSAAQRQ DPVRELQGTG SQLGGTTGEK LPAGRSKAEL RAERRAKQEA ERALKQARKG EQGGPSPQAC PSTAGEATSG VKRVPEHTQA DDPTLLRRLL RKPDRQQVPT RKDYGSKVSL FSHLPQYSRQ SSLTQYMSIP SSVIHPAMVR LGLQYSQGLV SGSNARCIAL LHALQQVIQD YTTPPNEELS RDLVNKLKPY ISFLTQCRPM SASMCNAIKF FNKEVTGMSS SKREEEAKSE LKEAIDRYVQ EKIVLASQAI SRFASKKISD GDVILVYGCS SLVSRILQEA WVEGRRFRVV VVDSRPRLEG RHMLHCLVRA GVPTSYLLIP AASYVLPEVS KVLLGAHALL ANGSVMSRVG TAQLALVARA HNVPVLVCCE TYKFCERVQT DAFVSNELDD PDDLQCKRGD QVTLANWQNN SSLRLLNLVY DVTPPELVDL VITELGMIPC SSVPVVLRVK SSDQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Gene References into Functions
  1. insulin-like growth factor 1-induced eIF2B activation in neurons is effected by protein phosphatase 1, the activation of which is mediated by the MEK/MAPK signaling pathway PMID: 12624094
  2. The eukaryotic initiation factor 2B beta, gamma, delta and epsilon subunit protein levels are maximal at embryonic day 18 and then decrease during development. Aged hippocampus contain only trace amounts of these subunits. PMID: 12850562
  3. Significant increases in the relative abundance of the mRNAs coding for the epsilon (33%) and gamma (26%) subunits of eukaryotic initiation factor (eIF) 2B were observed 48 h after the exercise bout. PMID: 14565967
Protein Families
EIF-2B alpha/beta/delta subunits family
Database Links

KEGG: rno:117019

UniGene: Rn.11060

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