Recombinant Rat Translation initiation factor eIF-2B subunit epsilon (Eif2b5)

Code CSB-YP720666RA
MSDS
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Source Yeast
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Code CSB-EP720666RA
MSDS
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Source E.coli
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Code CSB-EP720666RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP720666RA
MSDS
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Source Baculovirus
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Code CSB-MP720666RA
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
Eif2b5
Uniprot No.
Alternative Names
Eif2b5; Eif2beTranslation initiation factor eIF-2B subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon
Species
Rattus norvegicus (Rat)
Expression Region
1-716
Target Protein Sequence
MAATAAVPSA VGGRANKRGG GSGGGGTQGA EEEPPPPLQA VLVADSFDRR FFPISKDQPR VLLPLANVAL IDYTLEFLTA TGVQETFVFC CWKAAQIKEH LQKSKWCHPT SLNVVRIITS DLYRSLGDVL RDVDAKALVR SDFLLIYGDV VSNINISKAL EEHRLRRKLE KNVSVMTMVF KESSPSHPTR CHEDNVVLAV DSTTNRILHF QKTQGLRHFS FPLGLFQGSL DGVEIRYDLL DCHISICSPQ VAQLFTDNFD YQTRDDFVRG LLVNEEILGN QIHLHVTSRE YGARVSNLHM YSAVCADVIR RWVYPLTPEV NFTDSSTQSY THSRHNIYRG PEVSLGHGSV LEENVLLGAG TVVGSNCSIT NSVIGPNCHI GDNVVLDQAY LWQGVRVAAG AQIHQSLLCD RAEVKERVIL KPHCVLTSQV VVGPDIILPE GSVISLHPPD AEEDEDDGQF SDDSGADQEK EKVKLKGYNP AEVGPEGQGY LWKAEDVDEK EDEELRQSLW GLMINMEEES ETESERSVDP EELDSRAGSP QLDDIRVFQN EVLGTLQRGR EENISCDNLV LEINSLKYAY NISLKEVMQV LSHVVLEFPL QQVDGVLDPN RYCALLLPLL KAWSPVFRNY IKRAADHLEA LAAIEDFFLE HETLVPSLAK VLMAFYQLEI LAEETILSWF SQRDITDKGQ QLRKNQQLQR FIQWLREAEE ESSDDD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Gene References into Functions
  1. modulation of eIF2Bepsilon expression may be sufficient to correct deficits in skeletal muscle protein synthesis associated with sepsis and other muscle-wasting conditions PMID: 20484009
  2. oligodendroglial-derived cells expressing a mutated eIF2B5 constantly use their stress response mechanism as an adaptation mean in order to survive PMID: 19023445
Protein Families
EIF-2B gamma/epsilon subunits family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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