Recombinant Rat Tyrosine-protein kinase FRK (Frk)

Code CSB-YP730799RA
MSDS
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Source Yeast
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Code CSB-EP730799RA
MSDS
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Source E.coli
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Code CSB-EP730799RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP730799RA
MSDS
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Source Baculovirus
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Code CSB-MP730799RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Frk
Uniprot No.
Alternative Names
Frk; GaskTyrosine-protein kinase FRK; EC 2.7.10.2; FYN-related kinase; Gastrointestinal-associated kinase; GASK; Gastrointestinal-associated tyrosine kinase; GTK
Species
Rattus norvegicus (Rat)
Expression Region
1-506
Target Protein Sequence
MGSVCMRLWA YLQPFLPCWS QEADKSVVIE NPGAYCPPEA NRSQGQYFVA LFDYEARTAE DLSFHAGDKL QVLDTSHEGW WLARHLEKKG PGLGQQLQGY IPSNYVAEDR SLQAEPWFFG AIKRADAEKQ LLYSENQTGA FLIRESESQK GDFSLSVLDE GVVKHYRIRR LDEGGFFLTR RKTFSTLNEF VNYYTTTSDG LCVKLEKPCL KIQVPTPFDL SYKTVDQWEI DRNSIQLLKR LGSGQFGEVW EGLWNNTTPV AVKTLKPGSM DPNDFLREAQ IMKSLRHPKL IQLYAVCTLE DPIYIITELM RHGSLQEYLQ NDGGSKIRLT QQVDMAAQVA SGMAYLESQN YIHRDLAARN VLVGEHNIYK VADFGLARVF KVDNEDIYES KHEIKLPVKW TAPEAIRTNK FSIKSDVWSF GILLYEIITY GKMPYSGMTG AQVIHMLGQN YRLPQPSNCP EQFYSIMMEC WNVEPKQRPT FETLHWKLED YFEPDSSYSD TNNFIN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
Gene References into Functions
  1. The tyrosine kinases Brk/PTK6/Sik, Srm, Frk/Rak/Gtk/Iyk/Bsk, and Src42A/Dsrc41 have a low degree of sequence homology to other known kinases. The exon structure of these kinases, called the Brk family, is highly conserved and distinct. PMID: 12725532
  2. Fyn kinase is associated with sodium channels in brain neurons and can modulate Na(v)1.2 channels by tyrosine phosphorylation after activation of TrkB/p75 neurotrophin receptor signaling by brain-derived neurotrophic factor (BDNF). PMID: 17959796
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Protein kinase superfamily, Tyr protein kinase family, SRC subfamily
Tissue Specificity
Highly expressed in stomach, small intestine and colon. Concentrated in the brush border membranes of epithelial cells, throughout the maturation axis of the adult small intestine.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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