Recombinant Rat WD repeat-containing protein 1 (Wdr1)

Code CSB-YP725492RA
MSDS
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Source Yeast
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Code CSB-EP725492RA
MSDS
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Source E.coli
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Code CSB-EP725492RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP725492RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP725492RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Wdr1
Uniprot No.
Alternative Names
Wdr1WD repeat-containing protein 1
Species
Rattus norvegicus (Rat)
Expression Region
2-606
Target Protein Sequence
PYEIKKVFA SLPQVERGVS KILGGDPKGD NFLYTNGKCV ILRNIDNPAV ADIYTEHAHQ VVVAKYAPSG FYIASGDISG KLRIWDTTQK EHILKYEYQP FAGKIKDIAW TEDSKRIAVV GEGREKFGAV FLWDTGSSVG EITGHNKVIN SVDIKQNRPY RLATGSDDNC AAFFEGPPFK FKFTIGDHSR FVNCVRFSPD GNRFATASAD GQIFIYDGKT GEKVCALGGS KAHDGGIYAI SWSPDSTHLL SASGDKTSKI WDVNVNSVVS TFPMGSNVLD QQLGCLWQKD HLLSISLSGY INYLDKNNPS KPLRVIKGHS KSIQCLTVHK NGGKSYIYSG SHDGHINYWD SETGENDSFS GKGHTNQVSR MTVDESGQLV SCSMDDTVRY TNLTLRDYSG QGVVKLDVQP KCVAVGPGGY TVVVCIGQIV LLKDQKKCFS IDNPGYEPEV VAVHPGGDTV AVGGSDGNVR VYSILGATLK DEGKLLEAKG PVTDLAYSHD GAFLAVCDAS KVVTVFSVAD GYSENNVFYG HHAKIVCLAW SPDNEHFASG GMDMMVYVWT LSDPETKVKI QDAHRLHHVS SLAWLDEHTL VTTSHDASVK EWTITY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. Enhances cofilin-mediated actin severing. Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions. Involved in myocardium sarcomere organization. Required for cardiomyocyte growth and maintenance. Involved in megakaryocyte maturation and platelet shedding. Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension. Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt.
Gene References into Functions
  1. elevated WDR1 expression after acoustic overstimulation in the current experiments may provide a mechanism for protection against noise exposure. PMID: 22821633
Subcellular Location
Cytoplasm, cytoskeleton. Cell projection, podosome.
Protein Families
WD repeat AIP1 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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