Recombinant Rat Alpha-2 antiplasmin(Serpinf1)

Code CSB-EP021084RA
Size US$2466
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Uniprot No. Q80ZA3
Research Area Others
Species Rattus norvegicus (Rat)
Source E.coli
Expression Region 20-418aa
Target Protein Sequence QNVPDSSQDSPAPDSTGEPVVEEDDPFFKAPVNKLAAAVSNFGYDLYRLRSGAVSTGNILLSPLSVATALSALSLGAEQRTESVIHRALYYDLINNPDIHSTYKELLASVTAPEKNFKSASRIVFERKLRVKSSFVAPLEKSYGTRPRILTGNPRIDLQEINNWVQAQMKGKIARSTREMPSALSILLLGVAYFKGQWATKFDSRKTTLQDFHLDEDRTVRVPMMSDPKAILRYGLDSDLNCKIAQLPLTGSMSIIFFLPLTVTQNLTMIEESLTSEFVHDIDRELKTIQAVLTVPKLKLSYEGDVTNSLQDMKLQSLFESPDFSKITGKPVKLTQVEHRAAFEWNEEGAGTSSNPDLQPVRLTFPLDYHLNRPFIFVLRDTDTGALLFIGRILDPSST
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 71.4kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Citations

Pigment Epithelium-Derived Factor Increases Native Collateral Blood Flow to Improve Cardiac Function and Induce Ventricular Remodeling After Acute Myocardial Infarction. Liu X, et al,Journal of The American Heart Association,2019

Sample type: Human coronary artery endothelial cells
Application: In vitro bioactivity in cell culture
Review: HCAEC tube formation in Matrigel in response to pre-PEDF or to post-PEDF.
PMID: 31718448

PEDF improves cardiac function in rats subjected to myocardial ischemia/reperfusion injury by inhibiting ROS generation via PEDF‑R. Zhao Q.et al,Int J Mol Med,2018

Application: Drug related studies
Review: PEDF protects H9c2 cells against H/R-induced apoptosis. H9c2 cells were exposed to H/R conditions for various durations (0/0, 4/0, 4/2, 8/0, 8/2, 12/0 and 12/2 h). In addition, H9c2 cells treated with or without PEDF (10 nM) were exposed to normoxic or hypoxic/reoxygenation condition for 8/2 h.
PMID: 29532859

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