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| Code | CSB-E08819r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 87 | ||||
| Range % | 81-92 | |||||
| 1:2000 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4000 | Average % | 92 | ||||
| Range % | 85-97 | |||||
| 1:8000 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| The recovery of rat PEDFspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-95 | ||||
| EDTA plasma (n=4) | 87 | 83-91 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Rat PEDF ELISA Kit was designed for the quantitative measurement of Rat PEDF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
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Please provide the following information regarding Rat pigment epithelium-derived factor, PEDF ELISA Kit (CSB-E08819r):
a. Can cell culture lysates/supernatants samples be used with this kit? Have cell culture lysates/supernatants samples been tested with this kit?
b. If yes, what were the observed concentration values?
c. If they have not, would this assay be compatible with cell culture lysates/supernatants samples? Is there a recommended protocol to use with cell culture lysates/supernatants samples?
