Rat pigment epithelium-derived factor,PEDF ELISA Kit

Code CSB-E08819r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Rat PEDF ELISA Kit was designed for the quantitative measurement of Rat PEDF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Target Name pigment epithelium-derived factor,PEDF
Abbreviation PEDF
Uniprot No. Q80ZA3
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 87  
Range % 81-92  
1:2000 Average % 95  
Range % 91-99  
1:4000 Average % 92  
Range % 85-97  
1:8000 Average % 96  
Range % 91-102  
The recovery of rat PEDFspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 89-95  
EDTA plasma (n=4) 87 83-91  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.758 2.615 2.687 2.549  
200 2.065 1.968 2.017 1.879  
100 1.357 1.397 1.377 1.239  
50 0.846 0.831 0.839 0.701  
25 0.572 0.581 0.577 0.439  
12.5 0.354 0.334 0.344 0.206  
6.25 0.264 0.256 0.260 0.122  
0 0.139 0.137 0.138    
ELISA Data Analysis ELISA Standard Curve & Curve Expert software
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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Please provide the following information regarding Rat pigment epithelium-derived factor, PEDF ELISA Kit (CSB-E08819r):
a. Can cell culture lysates/supernatants samples be used with this kit? Have cell culture lysates/supernatants samples been tested with this kit?
b. If yes, what were the observed concentration values?
c. If they have not, would this assay be compatible with cell culture lysates/supernatants samples? Is there a recommended protocol to use with cell culture lysates/supernatants samples?

Thanks for your inquiry.
The kit can be used for cell culture supernatants matrix.
However, we have no detection on the sample. If the sample content is under the detection range, it can be detected well.
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