Rat pigment epithelium-derived factor,PEDF ELISA Kit

Rat pigment epithelium-derived factor,PEDF ELISA Kit

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Rat pigment epithelium-derived factor,PEDF ELISA Kit

Name: Rat pigment epithelium-derived factor,PEDF ELISA Kit
Brand: CUSABIO
SKU: CSB-E08819r

Citations (10) Protocol

Code	CSB-E08819r
Size	96T,5×96T,10×96T
Trial Size
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Product Details
Citations (10)
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Customer Reviews and Q&A

Description

This Rat PEDF ELISA Kit was designed for the quantitative measurement of Rat PEDF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Target Name

pigment epithelium-derived factor,PEDF

Abbreviation

PEDF

Uniprot No.

Q80ZA3

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	87
	Range %	81-92
1:2000	Average %	95
	Range %	91-99
1:4000	Average %	92
	Range %	85-97
1:8000	Average %	96
	Range %	91-102

Recovery

The recovery of rat PEDFspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-95
EDTA plasma (n=4)	87	83-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.758	2.615	2.687	2.549
200	2.065	1.968	2.017	1.879
100	1.357	1.397	1.377	1.239
50	0.846	0.831	0.839	0.701
25	0.572	0.581	0.577	0.439
12.5	0.354	0.334	0.344	0.206
6.25	0.264	0.256	0.260	0.122
0	0.139	0.137	0.138

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Application of rat cryptorchidism model for the evaluation of mesenchymal stromal cell secretome regenerative potential G.D.Sagaradze, et al,Biomedicine & Pharmacotherapy,2018
Pigment epithelium-derived factor attenuates myocardial fibrosis via inhibiting Endothelial-to-Mesenchymal Transition in rats with acute myocardial infarction Hao Zhang.et al,Scientific Reports,2017
A novel modified physiologically relevant model for cardiac angiogenesis Hao Zhang.et al,Microvasc Res,2017
PEDF and PEDF-derived peptide 44mer inhibit oxygen-glucose deprivation-induced oxidative stress through upregulating PPARr via PEDF-R in H9c2 cells. Wei Zhuang.et al,Biochem Biophys Res Commun.,2016
Pigment Epithelium-Derived Factor (PEDF) Improves Ischemic Cardiac Functional Reserve ThroughDecreasing Hypoxic Cardiomyocyte Contractility Through PEDF Receptor (PEDF-R). Lu P.et al,J Am Heart Assoc.,2016
PEDF and 34-mer inhibit angiogenesis in the heart by inducing tip cells apoptosis via up-regulating PPAR-r to increase surface FasL Zhang H. et al,Apoptosis,2015
PEDF Improves Cardiac Function in Rats with Acute Myocardial Infarction via Inhibiting Vascular Permeability and Cardiomyocyte Apoptosis Zhang H et al,Int J Mol Sci,2015
Influence of triazine herbicide exposure on guppies (poecilia sphenops) aromatase activities, altered sex steroid concentration and vitellogenin induction S Vasanth. et al,Indian J Pharm Sci.,2015
Comparison of the effects of letrozole and cabergoline on vascular permeability, ovarian diameter, ovarian tissue VEGF levels, and blood PEDF levels, in a rat model of ovarian hyperstimulation syndrome. ahin N. et al,Arch Gynecol Obstet,2015
PEDF and PEDF-derived peptide 44mer stimulate cardiac triglyceride degradation via ATGL Gao X et al,Sci Rep,2014

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■ Q&A

Please provide the following information regarding Rat pigment epithelium-derived factor, PEDF ELISA Kit (CSB-E08819r):
a. Can cell culture lysates/supernatants samples be used with this kit? Have cell culture lysates/supernatants samples been tested with this kit?
b. If yes, what were the observed concentration values?
c. If they have not, would this assay be compatible with cell culture lysates/supernatants samples? Is there a recommended protocol to use with cell culture lysates/supernatants samples?

Thanks for your inquiry.
The kit can be used for cell culture supernatants matrix.
However, we have no detection on the sample. If the sample content is under the detection range, it can be detected well.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.