Recombinant Rickettsia typhi Probable tRNA-dihydrouridine synthase (dus)

Code CSB-YP718158RNE
MSDS
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Source Yeast
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Code CSB-EP718158RNE
MSDS
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Source E.coli
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Code CSB-EP718158RNE-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP718158RNE
MSDS
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Source Baculovirus
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Code CSB-MP718158RNE
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
dus
Uniprot No.
Alternative Names
dus; RT0010Probable tRNA-dihydrouridine synthase; EC 1.3.1.-
Species
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Expression Region
1-327
Target Protein Sequence
MIKIGNIELS SNVILAPMSN ITDLEFRKLV KRFGAGLVVS EMIASRAMIM KSRQSMQKCA IMHDDPTSAC VQLAGCEPDV IADAAKMNED MGAKIIDLNF GCPAKKVVGG YAGSALMRDE RLATKIFEAT VKAVKIPVTV KMRMGWDDNT KNAPTLAVIA ASSGVQMVTV HGRTRCQFYS GNANWDFIRV VKEAVKIPVI ANGDITNFAK AKEALQKSGA DGIMVGRGVY GKPWLISQIA YYLKTGKEKP APSIAEQLDI IIKHYDAIID YYGKSVGVPI ARKHIIWYSS GLPSSAEFRC AVNLMNDPIA VKEKIAEFYM SVMDANK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.
Protein Families
Dus family
Database Links

KEGG: rty:RT0010

STRING: 257363.RT0010

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Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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