Recombinant Saccharomyces cerevisiae Hsp70 nucleotide exchange factor FES1 (FES1)

Code CSB-YP327807SVG
MSDS
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Source Yeast
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Code CSB-EP327807SVG
MSDS
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Source E.coli
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Code CSB-EP327807SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP327807SVG
MSDS
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Source Baculovirus
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Code CSB-MP327807SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FES1
Uniprot No.
Alternative Names
FES1; YBR101C; YBR0830Hsp70 nucleotide exchange factor FES1; Factor exchange for SSA1 protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-290
Target Protein Sequence
MEKLLQWSIA NSQGDKEAMA RAGQPDPKLL QQLFGGGGPD DPTLMKESMA VIMNPEVDLE TKLVAFDNFE MLIENLDNAN NIENLKLWEP LLDVLVQTKD EELRAAALSI IGTAVQNNLD SQNNFMKYDN GLRSLIEIAS DKTKPLDVRT KAFYALSNLI RNHKDISEKF FKLNGLDCIA PVLSDNTAKP KLKMRAIALL TAYLSSVKID ENIISVLRKD GVIESTIECL SDESNLNIID RVLSFLSHLI SSGIKFNEQE LHKLNEGYKH IEPLKDRLNE DDYLAVKYVL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in protein translation, propagation of [PSI+] prions, and polyamine tolerance. Functions as a nucleotide exchange factor (NEF), which accelerates the release of ADP, for the cytosolic Hsp70 chaperone SSA1 and the ribosome-associated Hsp70 chaperone SSB1. Required for fully efficient Hsp70-mediated folding of proteins.
Gene References into Functions
  1. Cytosolic splice isoform of Fes1 is required for the degradation of misfolded proteins in yeast.The FES1 transcript undergoes unique 3' alternative splicing that results in two equally active isoforms with alternative C-termini. PMID: 26912797
  2. cytosolic Hsp70 interacts with up to three types of nucleotide exchange factors (NEFs) homologous to human counterparts: Sse1/Sse2 (Heat shock protein 110 (Hsp110)), Fes1 (HspBP1), and Snl1 (Bag-1). PMID: 24671421
  3. Fes1, an Hsp70 nucleotide exchange factor of hitherto unclear physiological function, is a cytosolic triaging factor that promotes proteasomal degradation of misfolded proteins PMID: 23530227
  4. crystal structure and mechanism for Hsp70 nucleotide exchange PMID: 15694338
Subcellular Location
Cytoplasm.
Protein Families
FES1 family
Database Links

KEGG: sce:YBR101C

STRING: 4932.YBR101C

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