Recombinant Saccharomyces cerevisiae 26S protease subunit RPT4 (RPT4)

Code CSB-YP345691SVG
MSDS
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Source Yeast
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Code CSB-EP345691SVG
MSDS
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Source E.coli
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Code CSB-EP345691SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP345691SVG
MSDS
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Source Baculovirus
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Code CSB-MP345691SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RPT4
Uniprot No.
Alternative Names
RPT4; CRL13; PCS1; SUG2; YOR259C26S proteasome subunit RPT4; 26S protease subunit SUG2; Proteasomal cap subunit
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-437
Target Protein Sequence
SEEQDPLLA GLGETSGDNH TQQSHEQQPE QPQETEEHHE EEPSRVDPEQ EAHNKALNQF KRKLLEHRRY DDQLKQRRQN IRDLEKLYDK TENDIKALQS IGQLIGEVMK ELSEEKYIVK ASSGPRYIVG VRNSVDRSKL KKGVRVTLDI TTLTIMRILP RETDPLVYNM TSFEQGEITF DGIGGLTEQI RELREVIELP LKNPEIFQRV GIKPPKGVLL YGPPGTGKTL LAKAVAATIG ANFIFSPASG IVDKYIGESA RIIREMFAYA KEHEPCIIFM DEVDAIGGRR FSEGTSADRE IQRTLMELLT QMDGFDNLGQ TKIIMATNRP DTLDPALLRP GRLDRKVEIP LPNEAGRLEI FKIHTAKVKK TGEFDFEAAV KMSDGFNGAD IRNCATEAGF FAIRDDRDHI NPDDLMKAVR KVAEVKKLEG TIEYQKL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
Gene References into Functions
  1. Rpt4p and Cdc48p can extract some substrates from the ER in an independent, not co-operative, manner. PMID: 20519439
  2. Rpt4 works independently of Cdc48p in the ER-cytosol dislocation of ricin A chain PMID: 20519439
  3. The non-proteolytic requirement for the proteasomal Sug2 ATPase includes at least the heat and oxidative stress-responsive genes. PMID: 16517940
Protein Families
AAA ATPase family
Database Links

KEGG: sce:YOR259C

STRING: 4932.YOR259C

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