Recombinant Saccharomyces cerevisiae ATP-NADH kinase YEF1 (YEF1)

Code CSB-YP333717SVG
MSDS
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Source Yeast
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Code CSB-EP333717SVG
MSDS
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Source E.coli
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Code CSB-EP333717SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333717SVG
MSDS
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Source Baculovirus
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Code CSB-MP333717SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
YEF1
Uniprot No.
Alternative Names
YEF1; YEL041W; SYGP-ORF17; ATP-NADH kinase YEF1; EC 2.7.1.86
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-495
Target Protein Sequence
MKTDRLLINA SPETCTKGDA EMDTMDTIDR MTSVKVLAEG KVLSNFEEPG LMRCGYHDAK NWVRRLSSET IVGEDTSNLY PFYVDTAYDV RRLRKDLINA KVDLQVENLI IICNINDIST VFLMREVVEW ILRNFHSITV YVQDIFKKST QFAVGDLCKD SNCSKNRVKY WSKEFVKKHD SFFDLMITLG GDGTVLFASS IFTKDVPPIV PFALGSLGFL TNFEFQNFKE TLKHILTDEV RINLRMRLQC KLYRRNKPEI DAATGRKICY IDFISEHHVL NEVTIDRGPA PCLSLLELYG NDSLMTKVQG DGLIVATPTG STAYSLSAGG SLISPSVNAI AVTPICPHTL SFRPIILPDS MELKVRVDMN SRGTSWVNFD GKDRVELKQG DYVVITASPY SVPTIESSAS EFFESISKNL NWNDREEQKP FAHILSPKNQ EKYRLDSSKN GNDTISNPLE SSCISSDAQD EERKSVTETE TEIVVERTRQ AHFAI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
ATP-NADH kinase with a low phosphorylation activity of both NADH and NAD(+) to produce NADP and NADPH by using ATP. UTR1 is responsible for essentially all of the NAD/NADH kinase activity resident in the cytoplasm, whereas POS5 is responsible for all mitochondrial NAD/NADH kinase activity and consequent mitochondrial genome maintenance. YEF1 can substitute for UTR1 when overexpressed.
Protein Families
NAD kinase family
Database Links

KEGG: sce:YEL041W

STRING: 4932.YEL041W

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