Product Details

Purity

>85% (SDS-PAGE)

Target Names

YKU80

Uniprot No.

Q04437

Alternative Names

YKU80; HDF2; YMR106C; YM9718.05C; ATP-dependent DNA helicase II subunit 2; EC 3.6.4.12; ATP-dependent DNA helicase II subunit Ku80; High affinity DNA-binding factor subunit 2; Yeast Ku80

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Appears to have a role in recruitment of telomerase and CDC13 to the telomere and the subsequent telomere elongation. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.

Gene References into Functions

the yKu-distal portion of telomeres is bound by Rap1, which in turn reduces the potential for yKu(Yku80 and Yku70 )to mediate NHEJ. These results thus propose a solution to a long-standing conundrum, namely how to accommodate the apparently conflicting functions of Ku on telomeres. PMID: 27930670
Deletion of Est1 has a much greater impact on telomere lengthening in the presence of a Cdc13-Est2 fusion than deletion of YKU80, suggesting that any contribution that Ku may have in telomerase activation is minor compared to that of Est1. PMID: 24879463
The TORC1 signal is transduced by the Gln3/Gat1/Ure2 pathway, which controls the levels of the Ku heterodimer, a telomere regulator. PMID: 22169538
These findings contradict the recruitment model of Ku binding both DNA and RNA simultaneously and lead to a new model for the mechanism by which Ku's interaction with TLC1 RNA contributes to telomerase recruitment. PMID: 22365814
Ku must load directly onto the chromosome end in order to mediate its telomeric functions PMID: 21852961
The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation. [YKU80] PMID: 21441303
The authors show here that the essential Cdc13-Stn1-Ten1 complex is entirely dispensable for telomere protection in non-dividing cells, however, Yku and Rap1 become crucially important for this function in these cells. PMID: 20628356
Findings support the hypothesis that yKu and core X play a pivotal role in maintaining genome stability through nuclear architecture by mediating a defensive fold-back structure at yeast chromosome ends. PMID: 19652176
Findings implicate a role for both yKu and core X in stabilizing the genome against recombination events involving telomeric sequences. PMID: 19652177
Ku- and Esc1-dependent pathways mediate natural telomere anchoring in vivo PMID: 15014445
data support a model in which Ku recruits telomerase to telomeres in G1 phase when telomerase is inactive and promotes telomerase-mediated telomere lengthening in late S phase PMID: 15531893
yKu80 regulates the function of recombination enhancer during yeast mating type switching PMID: 16166630
Ku functions as a genomic gatekeeper through its crosstalk with DNA damage checkpoints PMID: 16446442
Mre11 functions in Rad51-independent break-induced replication (BIR), and Ku functions in Rad51-dependent BIR PMID: 17321803
A 'two-face' model for Ku is proposed and the divergent evolution of these faces allowed Ku's dual role in nonhomologous end-joining and telomere maintenance. PMID: 17351632
The Ku complex in silencing the cryptic mating-type loci of Saccharomyces cerevisiae. PMID: 18716325
Yku80-Dnl4 interactions are important for formation of a productive ligase-DSB intermediate. PMID: 18832348
Association of the Ku heterodimer with broken DNA ends inhibits recombination in mrx mutants, but not in repair-proficient cells or in other DNA repair single mutants. PMID: 18992851
yKu complex is associated with mating type locus HML and HMR sequences in a mating-type-specific manner. PMID: 19047366

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Subcellular Location

Nucleus. Chromosome, telomere.

Protein Families

Ku80 family

Database Links

KEGG: sce:YMR106C

STRING: 4932.YMR106C

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Code	CSB-YP311992SVG
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Size	Pls inquire
Source	Yeast
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Code	CSB-EP311992SVG
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Code	CSB-EP311992SVG-B
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Code	CSB-BP311992SVG
MSDS	MSDS
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Code	CSB-MP311992SVG
MSDS	MSDS
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Source	Mammalian cell
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Recombinant Saccharomyces cerevisiae ATP-dependent DNA helicase II subunit 2 (YKU80), partial

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