Recombinant Saccharomyces cerevisiae Actin-related protein 7 (ARP7)

Code CSB-YP619597SVG
MSDS
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Source Yeast
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Code CSB-EP619597SVG
MSDS
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Source E.coli
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Code CSB-EP619597SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP619597SVG
MSDS
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Source Baculovirus
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Code CSB-MP619597SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARP7
Uniprot No.
Alternative Names
ARP7; SWP61; YPR034W; YP9367.14Actin-related protein 7; Actin-like protein ARP7; Chromatin structure-remodeling complex protein ARP7; SWI/SNF complex component ARP7
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-477
Target Protein Sequence
MTLNRKCVVI HNGSHRTVAG FSNVELPQCI IPSSYIKRTD EGGEAEFIFG TYNMIDAAAE KRNGDEVYTL VDSQGLPYNW DALEMQWRYL YDTQLKVSPE ELPLVITMPA TNGKPDMAIL ERYYELAFDK LNVPVFQIVI EPLAIALSMG KSSAFVIDIG ASGCNVTPII DGIVVKNAVV RSKFGGDFLD FQVHERLAPL IKEENDMENM ADEQKRSTDV WYEASTWIQQ FKSTMLQVSE KDLFELERYY KEQADIYAKQ QEQLKQMDQQ LQYTALTGSP NNPLVQKKNF LFKPLNKTLT LDLKECYQFA EYLFKPQLIS DKFSPEDGLG PLMAKSVKKA GASINSMKAN TSTNPNGLGT SHINTNVGDN NSTASSSNIS PEQVYSLLLT NVIITGSTSL IEGMEQRIIK ELSIRFPQYK LTTFANQVMM DRKIQGWLGA LTMANLPSWS LGKWYSKEDY ETLKRDRKQS QATNATN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in transcriptional regulation. Heterodimer of ARP7 and ARP9 functions with HMG box proteins to facilitate proper chromatin architecture. Heterodimer formation is necessary for assembly into RSC complex. Part of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.
Subcellular Location
Nucleus. Note=Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.
Protein Families
Actin family
Database Links

KEGG: sce:YPR034W

STRING: 4932.YPR034W

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