Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 1 (AIM1)

Code CSB-YP331066SVG
MSDS
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Source Yeast
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Code CSB-EP331066SVG
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Source E.coli
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Code CSB-EP331066SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP331066SVG
MSDS
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Source Baculovirus
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Code CSB-MP331066SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BOL3
Uniprot No.
Alternative Names
BOL3; AIM1; YAL046CBolA-like protein 3; Altered inheritance of mitochondria protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-118
Target Protein Sequence
MKLPQTMLRS ISVKHVRWPR ILTGSKLWYS TQMAMTPEEK MITDKLQQEL EPEVCKVQDV SGGCGSMFAI NITSKKFNGL SLIKQHQLVN RILRDDISRW HGLQLTTKKS TGKGPASS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates [4Fe-4S] cluster insertion into a subset of mitochondrial proteins such as lipoyl synthase (LS) and succinate dehydrogenase (SDH). Required during the last step of iron-sulfur protein assembly when the iron-sulfur cluster is inserted into the target protein. Acts together with NFU1, later than BOL1 and GRX5 in the [4Fe-4S] cluster insertion process. Not required for [2Fe-2S] cluster insertion into mitochondrial proteins.
Gene References into Functions
  1. Bol1 and Bol3 form dimeric complexes with both monothiol glutaredoxin Grx5 and Nfu1. PMID: 27532772
  2. Additional studies focused on the mitochondrial BolA proteins, Bol1 and Bol3 (yeast homolog to human BOLA3), revealing that Bol1 functions earlier in iron-sulfur biogenesis with the monothiol glutaredoxin, Grx5, and Bol3 functions late with Nfu1. PMID: 27532773
Subcellular Location
Mitochondrion matrix.
Protein Families
BolA/IbaG family
Database Links

KEGG: sce:YAL046C

STRING: 4932.YAL046C

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