Recombinant Saccharomyces cerevisiae Aromatic/aminoadipate aminotransferase 1 (ARO8)

Code CSB-YP346818SVG
MSDS
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Source Yeast
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Code CSB-EP346818SVG
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Source E.coli
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Code CSB-EP346818SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP346818SVG
MSDS
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Source Baculovirus
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Code CSB-MP346818SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARO8
Uniprot No.
Alternative Names
ARO8; YGL202W; Aromatic/aminoadipate aminotransferase 1; 2-aminoadipate aminotransferase; EC 2.6.1.39; 2-aminoadipate transaminase; Alpha-aminoadipate aminotransferase; AadAT; Aromatic amino acid aminotransferase 1; EC 2.6.1.57; Aromatic amino acid aminotransferase I; Aromatic amino acid-requiring protein 8
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-500
Target Protein Sequence
MTLPESKDFS YLFSDETNAR KPSPLKTCIH LFQDPNIIFL GGGLPLKDYF PWDNLSVDSP KPPFPQGIGA PIDEQNCIKY TVNKDYADKS ANPSNDIPLS RALQYGFSAG QPELLNFIRD HTKIIHDLKY KDWDVLATAG NTNAWESTLR VFCNRGDVIL VEAHSFSSSL ASAEAQGVIT FPVPIDADGI IPEKLAKVME NWTPGAPKPK LLYTIPTGQN PTGTSIADHR KEAIYKIAQK YDFLIVEDEP YYFLQMNPYI KDLKEREKAQ SSPKQDHDEF LKSLANTFLS LDTEGRVIRM DSFSKVLAPG TRLGWITGSS KILKPYLSLH EMTIQAPAGF TQVLVNATLS RWGQKGYLDW LLGLRHEYTL KRDCAIDALY KYLPQSDAFV INPPIAGMFF TVNIDASVHP EFKTKYNSDP YQLEQSLYHK VVERGVLVVP GSWFKSEGET EPPQPAESKE VSNPNIIFFR GTYAAVSPEK LTEGLKRLGD TLYEEFGISK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
General aromatic amino acid transaminase involved in several otherwise unrelated metabolic pathways. Responsible for phenylalanine and tyrosine biosynthesis. Active with glutamate, phenylalanine, tyrosine and tryptophan as amino donors and with phenylpyruvate, hydroxyphenylpyruvate, 2-oxoglutarate and pyruvate as amino acceptors. Also active with methionine, alpha-aminoadipate and leucine as amino donors when phenylpyruvate is the amino acceptor and in the reverse reactions with the corresponding oxo acids and phenylalanine as amino donor. Catalyzes the formation of methionine from 2-keto-4-methylthiobutyrate (KMTB) in the methionine salvage pathway primarily using aromatic amino acids (tyrosine, phenylalanine and tryptophan) as the amino donors. Catalyzes the formation of alpha-aminoadipate from alpha-ketoadipate in the lysine biosyntheic pathway.
Gene References into Functions
  1. Deletion of ARO8 led to de novo production of phenylethanol during growth on a glucose synthetic medium. PMID: 24733517
  2. The aromatic aminotransferase Aro8 has been identified as an AAA-AT in Saccharomyces cerevisiae. This enzyme displays broad substrate selectivity, utilizing several amino acids and 2-oxo acids as substrates. PMID: 23893908
Subcellular Location
Cytoplasm.
Protein Families
Class-I pyridoxal-phosphate-dependent aminotransferase family
Database Links

KEGG: sce:YGL202W

STRING: 4932.YGL202W

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