Recombinant Saccharomyces cerevisiae Autophagy-related protein 34 (ATG34)

Code CSB-YP622459SVG
MSDS
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Source Yeast
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Code CSB-EP622459SVG
MSDS
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Source E.coli
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Code CSB-EP622459SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP622459SVG
MSDS
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Source Baculovirus
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Code CSB-MP622459SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ATG34
Uniprot No.
Alternative Names
ATG34; ATG19-B; YOL083W; O0957Autophagy-related protein 34
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-412
Target Protein Sequence
MKIAVETTLF DFFVIDQFKK STFSAPNTKV DTIKGCINKF IEQFNVYDEQ HIFWQPPGKS NVRLLSNAND FGQLGNFLHK KIKCNIFIGE EALRKYDLNI CGPYDKFVEN SDPSVKKVVN RDDVMLSRKC LNIISEQLSI LEKSISKAQN QVLQSSEVEG KKCIILPEDK PELIKFFSKF ETSVQLQEVY EGYKVYEKLL QKFGGQKKRM ESFLNENTPM SGAEAIKQIN ISEELKEKGE RLTTPNDPLL HVEVSNEDNS LHFILYNKTN IIIPGNCTFE FSSQISEVFS IKMGPHEIGI KGQKELWFFP SLPTPLSNYT MKVVNQDGET ILVGKCADSN EITLKSPLAS FSTGSFQTGS FHTLQDPTNV FRADALSSPD ESSIMSTPFL GETDEVYNSG STLSRPFTWE EI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cargo-receptor protein involved in the cytoplasm to vacuole transport (Cvt) and in autophagy. Recognizes cargo proteins, such as AMS1 and delivers them to the pre-autophagosomal structure for eventual engulfment by the autophagosome and targeting to the vacuole.
Gene References into Functions
  1. Hrr25 as the kinase that phosphorylates to promote vacuolar transport of alpha-mannosidase Atg34 PMID: 25281559
Subcellular Location
Preautophagosomal structure membrane; Peripheral membrane protein. Note=Membrane-associated protein found in pre-autophagosomal structure and other perivacuolar punctate structures.
Database Links

KEGG: sce:YOL083W

STRING: 4932.YOL083W

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