Recombinant Saccharomyces cerevisiae Bud neck protein 5 (BNI5)

Code CSB-YP344917SVG
MSDS
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Source Yeast
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Code CSB-EP344917SVG
MSDS
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Source E.coli
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Code CSB-EP344917SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344917SVG
MSDS
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Source Baculovirus
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Code CSB-MP344917SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BNI5
Uniprot No.
Alternative Names
BNI5; YNL166C; N1706Bud neck protein 5
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-448
Target Protein Sequence
MGLDQDKIKK RLSQIEIDIN QMNQMIDENL QLVEPAEDEA VEDNVKDTGV VDAVKVAETA LFSGNDGADS NPGDSAQVEE HKTAQVHIPT ENEANKSTDD PSQLSVTQPF IAKEQITHTA IAIGDSYNSF VANSAGNEKA KDSCTENKED GTVNIDQNRG EADVEIIENN DDEWEDEKSD VEEGRVDKGT EENSEIESFK SPMPQNNTLG GENKLDAELV LDKFSSANKD LDIQPQTIVV GGDNEYNHES SRLADQTPHD DNSENCPNRS GGSTPLDSQT KIFIPKKNSK EDGTNINHFN SDGDGQKKMA NFETRRPTNP FRVISVSSNS NSRNGSRKSS LNKYDSPVSS PITSASELGS IAKLEKRHDY LSMKCIKLQK EIDYLNKMNA QGSLSMEDGK RLHRAVVKLQ EYLDKKTKEK YEVGVLLSRH LRKQIDRGEN GQFWIGTK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for normal septin function and cytokinesis.
Gene References into Functions
  1. These results suggest that bud morphogenesis and mitotic entry are positively regulated by phosphorylation-dependent function of Bni5 which is under the control of Swe1 morphogenesis pathway. PMID: 17342053
Subcellular Location
Cytoplasm. Bud. Note=Arrives at the bud site approximately coincident with bud emergence and dissociates from the septin scaffold before cytokinesis.
Database Links

KEGG: sce:YNL166C

STRING: 4932.YNL166C

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