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Recombinant Saccharomyces cerevisiae Bud site selection protein 6 (BUD6), partial

Recombinant Saccharomyces cerevisiae Bud site selection protein 6 (BUD6), partial

Code	CSB-YP328288SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP328288SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP328288SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP328288SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP328288SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

BUD6

Uniprot No.

P41697

Alternative Names

BUD6; AIP3; YLR319C; L8543.5Bud site selection protein 6; Actin-interacting protein 3

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Not essential for mitotic growth but is necessary for normal morphogenesis. Involved in the organization and/or function of the actin cytoskeleton.

Gene References into Functions

Structure of a Bud6/Actin complex reveals a novel wh2-like actin monomer recruitment motif. PMID: 26118535
Yor304c-a/Bil1 colocalizes with Bud6 and functions in the Bnr1 pathway for actin assembly PMID: 23671312
model in which a Bni1/Bud6 complex with a 2:4 subunit stoichiometry assembles a nucleation seed with Bud6 coordinating up to four actin subunits PMID: 23161908
Bud6p and Kip3p play antagonistic roles in controlling the length of microtubules contacting the bud. PMID: 22608510
Bud6 promotes actin filament nucleation by recruiting actin monomers to Bni1 PMID: 21880892
Bud6p has a role in MT capture at the cell cortex, independent of Kar9p-mediated MT delivery along actin cables PMID: 15492045
Bni1 and Bnr1 are regulated by Bud6 PMID: 15923184
Bud6p, through its localization at both spindle pole bodies and at the mother-bud neck, provides a regulatory mechanism to prevent excessive oscillations of preanaphase nuclei, thus reducing the likelihood of mitotic delays and nuclear missegregation. PMID: 17416900
BNI1 and BNR1 contribute to Bud6p-dependent microtubule-cortex interactions by controlling Bud6p localisation. PMID: 18957510