Recombinant Saccharomyces cerevisiae Chromosome segregation in meiosis protein 3 (CSM3)

Code CSB-YP312018SVG
MSDS
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Source Yeast
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Code CSB-EP312018SVG
MSDS
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Source E.coli
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Code CSB-EP312018SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP312018SVG
MSDS
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Source Baculovirus
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Code CSB-MP312018SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CSM3
Uniprot No.
Alternative Names
CSM3; YMR048W; YM9796.01Chromosome segregation in meiosis protein 3
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-317
Target Protein Sequence
MDQDFDSLLL GFNDSDSVQK DPTVPNGLDG SVVDPTIADP TAITARKRRP QVKLTAEKLL SDKGLPYVLK NAHKRIRISS KKNSYDNLSN IIQFYQLWAH ELFPKAKFKD FMKICQTVGK TDPVLREYRV SLFRDEMGMS FDVGTRETGQ DLERQSPMVE EHVTSAEERP IVADSFAQDK RNVNNVDYDN DEDDDIYHLS YRNRRGRVLD ERGNNETVLN NVVPPKEDLD ALLKTFRVQG PVGLEENEKK LLLGWLDAHR KMEKGSMTEE DVQLIQSLEE WEMNDIEGQH THYDLLPGGD EFGVDQDELD AMKEMGF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Forms a fork protection complex (FPC) with TOF1 which is required for chromosome segregation during meiosis and DNA damage repair. FPC coordinates leading and lagging strand synthesis and moves with the replication fork. FPC stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors and protects stalled replication forks against the fork-releasing activity of RRM3 helicase.
Gene References into Functions
  1. although fork rotation and precatenation facilitate unwinding in hard-to-replicate contexts, they intrinsically disrupt normal chromosome duplication and are therefore restricted by Timeless/Tipin PMID: 26240319
  2. The replication stalling complex, Mrc1/Tof1/Csm3, reduces CAG repeat contraction rates independently of replication checkpoint and DNA damage checkpoint factors. PMID: 18321795
  3. Ctf19 and Csm3 are both important mediators of pericentromeric cohesion. PMID: 19730685
Subcellular Location
Nucleus.
Protein Families
CSM3 family
Database Links

KEGG: sce:YMR048W

STRING: 4932.YMR048W

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