Recombinant Saccharomyces cerevisiae Cytochrome b translational activator protein CBS1, mitochondrial (CBS1)

Code CSB-YP319640SVG
MSDS
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Source Yeast
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Code CSB-EP319640SVG
MSDS
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Source E.coli
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Code CSB-EP319640SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP319640SVG
MSDS
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Source Baculovirus
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Code CSB-MP319640SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CBS1
Uniprot No.
Alternative Names
CBS1; YDL069C; Cytochrome b translational activator protein CBS1; mitochondrial; COB-specific translational activator protein CBS1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
26-229
Target Protein Sequence
INTVT KKNISKIEKL CEVLEVNPDG YKGKERIPTK ELTKLLYTTS RNMLVRVPMT GDLSTGNTFE TRNETLQKLG EQLIHLEINK MLTITFTNFN QFNIMNKNFN YIHNLDRARV VNMDSISWLI KNSLKINQLA HLRIPANLPK EMGLTSSSND FQNLNDWKVI LSFIGYLKLL EIKNDNKKFI ESIIKTICIP LINYHLRKS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
mRNA-specific translational activator of cytochrome b. The cytochrome b (COB) leader RNA may represent the target sequence for CBS1 and CBS2, tethering the COB mRNA to the inner mitochondrial membrane, where cotranslational insertion of cytochrome b into the membrane can occur.
Gene References into Functions
  1. Cbs1p is associated with mitochondrial ribosomes; the observed interaction is mediated by ribosome-bound mRNA, thus restricting the association to ribosomes actively translating cytochrome b PMID: 15927884
Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein.
Database Links

KEGG: sce:YDL069C

STRING: 4932.YDL069C

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