Recombinant Saccharomyces cerevisiae DNA damage-inducible protein DIN7 (DIN7)

Code CSB-YP606294SVG
MSDS
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Source Yeast
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Code CSB-EP606294SVG
MSDS
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Source E.coli
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Code CSB-EP606294SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP606294SVG
MSDS
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Source Baculovirus
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Code CSB-MP606294SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DIN7
Uniprot No.
Alternative Names
DIN7; DIN3; YDR263C; YD9320B.02CDNA damage-inducible protein DIN7; EC 3.1.-.-
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-430
Target Protein Sequence
MGIPGLLPQL KRIQKQVSLK KYMYQTLAID GYAWLHRASC ACAFELVMNK PTNKYLQFFI KRLQLLKRLK IKPYIVFDGD SLFVKNHTET RRRKKRLENE MIAKKLWSAG NRYNAMEYFQ KSVDITPEMA KCIIDYCKLH SIPYIVAPFE ADPQMVYLEK MGLIQGIISE DSDLLVFGCK TLITKLNDQG KALEISKDDF SALPENFPLG ELSEQQFRNL VCLAGCDYTS GIWKVGVVTA MKIVKRYSEM KDILIQIERT EKLCFSKAFK QQVEFANYAF QYQRVFCPLS NQITTLNNIP KAVTNSHAEI IKIMKCIGSV VERGSGVRKD VINTKNIDHK VHEMIAKGEL HPVDMASKLI NRERKLKARK LFKVGLLGGE SNSFNKKVEQ PLVDTQDVLS ERENSLDNKN ASSIYMTSPA AISGTVPSIF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
5'->3' double-stranded DNA exonuclease.
Gene References into Functions
  1. Genotoxicity of chemical compounds identification and assessment by yeast cells transformed with GFP reporter constructs regulated by the PLM2 or DIN7 promoter PMID: 25691521
  2. The results suggest that after homologous pairing, the relative activity levels of Din7 and Mhr1 modulate the preference for replication versus homologous recombination to repair double-strand-breaks at ori5. PMID: 23598996
  3. The putative recombination genes CCE1, DIN7, and MHR1 have overlapping function with the mismatch repair homolog MSH1 in point mutation avoidance and suppression of aberrant recombination events. PMID: 16337661
  4. 19-bp sequence is a novel cis-acting element that is required for the regulation of DIN7 expression in response to HU-induced DNA damage PMID: 17988640
Subcellular Location
Nucleus.
Protein Families
XPG/RAD2 endonuclease family
Database Links

KEGG: sce:YDR263C

STRING: 4932.YDR263C

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