Recombinant Saccharomyces cerevisiae DNA endonuclease SAE2 (SAE2)

1. MRX-Sae2 efficiently cleaves near Ku-blocked DNA ends, which shows how recombination competes with NHEJ in the S/G2 phase when Sae2 is phosphorylated and thus capable of promoting endonucleolytic DNA cleavage by MRX PMID: 29321179
2. Authors find that the ability of Sae2 to promote MRX nuclease functions is important for DNA damage survival, particularly in the absence of Dna2 nuclease activity. PMID: 28970327
3. CtIP/Ctp1/Sae2/Com1 role in removal of DNA double strand breaks through DSB repair by homologous recombination is reviewed. PMID: 28623092
4. our data suggest a direct role for Sae2 nuclease activity in processing of the DNA structures that arise during replication and DNA damage and provide insights into the mechanism underlying Mre11-Sae2-mediated abrogation of replication stressrelated defects in S. cerevisiae. PMID: 27966484
5. DNA end resection by Sae2 and Sgs1 is dispensable for normal telomere maintenance by telomerase. PMID: 25254351
6. without Sae2 or Mre11 nuclease activity, Mre11 bound to partly processed DSBs impairs strand invasion and HR. PMID: 25899817
7. threonine-specific phosphorylation of Sae2 by Mec1 and Tel1 contributes to DNA repair and genome maintenance via its interactions with Rad53 and Dun1 PMID: 25762720
8. We found that Sae2 sumoylation occurs independently of its phosphorylation, and the two modifications act in synergy to increase soluble forms of Sae2. PMID: 25569253
9. DNA damage also triggers removal of Sae2 through autophagy. PMID: 24344201
10. we provide direct molecular characterization of coincident resection at random, radiation-induced DSBs and show that rapid and coincident initiation of resection at DSBs requires MRX, Sae2 protein, and Mre11 nuclease PMID: 23555316
11. Lif1 phosphorylation at Ser261 is probably involved in micro-homology-dependent end joining associated with producing single-stranded DSB ends that are formed by Sae2 as early intermediates in the homologous recombination pathway. PMID: 22563681
12. Data show that Mre11p and Sae2p are not required for DSB formation at FS2 or the subsequent repair of these DSBs. PMID: 19635935
13. participates in DSB single strand annealing repair by ensuring both resection and intrachromosomal association of the broken ends PMID: 16162495
14. checkpoint-mediated phosphorylation of Sae2 is important to support its meiotic recombination functions PMID: 16861895
15. Results idientify COM1, a novel plant gene essential for meiosis that is related to the human CtIP and the yeast COM1/SAE2 gene. PMID: 18007598
16. Sae2 oligomerizes independently of DNA damage and that oligomerization is required for its regulatory influence on the Mre11 nuclease and checkpoint functions. PMID: 18245357
17. Phosphorylation of the three C-terminal phosphorylation sites of Sae2 is necessary for DNA double-strand break end resection. PMID: 18670132
18. cell-cycle control of double strand break resection in Saccharomyces cerevisiae results from the phosphorylation by CDK of an evolutionarily conserved motif in the Sae2 protein PMID: 18716619
19. When Sae2 is absent, in addition to Exo1 and Sgs1, unprocessed DSBs accumulate and homology-dependent repair fails PMID: 18806779
20. Data show tha Sae2 and the Sgs1 RecQ helicase control two distinct but partially complementary pathways for nucleolytic processing telomeres, with Sae2 function requiring its serine 267 phosphorylation. PMID: 19595717

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KEGG: sce:YGL175C

STRING: 4932.YGL175C