Recombinant Saccharomyces cerevisiae DNA polymerase delta catalytic subunit (POL3), partial

1. to the synthesis of both nascent strands immediately at origins, likely by initiating leading-strand replication PMID: 29487291
2. Induced and spontaneous mutagenesis in pol3-13 mutant yeast strains and its dependence on theREV3 gene, encoding the catalytic subunit of pol delta were studied. The defect in coordination of the Fe-S cluster in pol delta conferred by the pol3-13 mutation abolishes UV-mutagenesis, but found that it elevates the incidence of spontaneous Rev3-dependent base-substitution mutations and deletions. PMID: 28034630
3. The effect of mutations in beta hairpin structure in POL3 on its proofreading DNA polymerase 3'-->5' exonuclease activity is studied. PMID: 25753811
4. Study finds that wild-type Pol epsilon molecules cannot proofread errors created by proofreading-defective Pol epsilon molecules, whereas Pol delta can not only proofread errors created by proofreading-defective Pol delta molecules, but can also proofread errors created by Pol epsilon-defective molecules. PMID: 25742645
5. genetic studies with Pol3 and Pol2 mutator alleles support the conclusion that Poldelta, and not Polepsilon, is the major DNA polymerase for carrying out both leading and lagging DNA synthesis PMID: 26145172
6. Poldelta-R696W promotes its own infidelity by expansion of dNTP pools PMID: 25827231
7. Data suggest that DNA-directed DNA polymerase Pols alpha and delta are the primary lagging-strand replicases and Pol epsilon is primarily a leading-strand replicase. PMID: 25217194
8. TLS polymerase REV1 carries out DNA lesion bypass only after the Def1-assisted removal of Pol3 from the stalled replication fork. PMID: 24465179
9. the initial steps of break-induced replication occur normally in Pif1-deficient cells, but Poldelta recruitment and DNA synthesis are decreased, resulting in premature resolution of DNA intermediates into half-crossovers PMID: 24025768
10. A substitution in the fingers domain of DNA polymerase delta reduces fidelity by altering nucleotide discrimination in the catalytic site PMID: 23283971
11. Data suggest the existence of a small number of additional sites in the yeast genome that interact with Pol III transcription complexes. PMID: 22287102
12. Loss of the last four amino acids at the C-terminal end of Pol3 impairs the interaction with Pol31. Pol delta stability is impaired in the pol3-ct mutant. PMID: 20813592
13. the 3'-->5' exonuclease of Saccharomyces cerevisiae DNA polymerase delta has three biological functions that require switching between the polymerase and exonuclease domains PMID: 15601866
14. These results argue strongly for the preferential recruitment of Poldelta during homologous recombination. PMID: 18086882
15. exchange of Saccharomyces cerevisiae Poldelta with Poleta requires both the stalling of the holoenzyme and the monoubiquitination of proliferating cell nuclear antigen PMID: 18385374
16. These data support a model of the replication fork wherein the leading and lagging strand templates are primarily copied by Pol epsilon and Pol delta, respectively. PMID: 18439893
17. Reduced levels of Pol3p, the catalytic subunit of DNA polymerase delta, induce instability at these same sites and lead to the generation of a variety of chromosomal aberrations. PMID: 18591249
18. our genetic evidence supports a novel role of Pol3 in nonhomologous end joining that facilitates gap filling at 3' overhangs in the absence of Pol4 to maintain genomic integrity. PMID: 18606574
19. DNA polymerase delta is highly processive with proliferating cell nuclear antigen and undergoes collision release upon completing DNA PMID: 18635534
20. lack of coordination between DNA polymerase delta and Rad27/Fen1 with the PCNA sliding clamp during long-patch base excision repair of alkylation damage can result in many double-strand breaks within the chromosomes of nondividing haploid cells PMID: 19075004
21. the BIR defect observed for rad51 mutants is due to strand invasion failure, whereas the Pol delta complex mutants are proficient for strand invasion but unable to complete extensive tracts of recombination-initiated DNA synthesis. PMID: 19139272
22. Study concludes that hmo1Delta suppression of the DNA pol delta mutation, pol3-14, occurs by a mechanism whereby normal controls on DNA integrity are breached and lesions flow into RAD52-mediated repair and error-prone pathways. PMID: 19184026
23. Crystal structure Pol delta in ternary complex with a template primer and incoming nucleotide catches the enzyme in the act of replication; polymerase and exonuclease domains are juxtaposed relative to each other & nucleotide is selected & incorporated. PMID: 19718023
24. propose an orientation for the accessory Pol31-Pol32N subunits relative to the Pol3 catalytic core that best agrees with the experimental scattering profile PMID: 19818796
25. The POL3 allele, pol3-t, of Saccharomyces cerevisiae, hyperrecombination phenotype and DNA damage-induced recombination is RAD50 dependent. PMID: 19834566
26. PCNA has a specific role in the initiation of recombination-associated DNA synthesis and that DNA polymerase delta promotes recombination-associated DNA synthesis. PMID: 19941829

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KEGG: sce:YDL102W

STRING: 4932.YDL102W