Recombinant Saccharomyces cerevisiae Defective in cullin neddylation protein 1 (DCN1)

Code CSB-YP619594SVG
MSDS
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Source Yeast
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Code CSB-EP619594SVG
MSDS
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Source E.coli
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Code CSB-EP619594SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP619594SVG
MSDS
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Source Baculovirus
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Code CSB-MP619594SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DCN1
Uniprot No.
Alternative Names
DCN1; YLR128W; L3111Defective in cullin neddylation protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-269
Target Protein Sequence
MSNNKIKRKD ASPEQEAIES FTSLTKCDPK VSRKYLQRNH WNINYALNDY YDKEIGTFTD EVSTVAHPPV YPKELTQVFE HYINNNLFDI DSLVKFIEEL GYNLEDLATL CLAHLLGYKK LEEPLKREDF LSTWFMQGCS TISDMQECIK TLDVKLHEDL QYFTQIYNYA FNLILDPNRK DIDTDEGIQY WKLFFQPEYP VRMEPDLLEA WFRFLRDEGK TTISKDTWRM LLLFFKRYPT IQKIISDYDE TAAWPFIIDE FYECLQDQQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes. Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity. Does not act by preventing deneddylation, but rather facilitates neddylation, possibly by acting with HRT1/RBX1 to recruit the Nedd8-charged E2 UBC12 to the cullin component of SCF-type complexes.
Gene References into Functions
  1. Both in vivo and in vitro experiments indicate that Dcn1p does not inhibit deneddylation of Cdc53p by the COP9 signalosome, but greatly increases the kinetics of the neddylation reaction PMID: 15988528
  2. DCN-1 is a scaffold protein in a multisubunit neddylation E3 ligase complex PMID: 17597076
  3. The 1.9 A X-ray crystal structure of Dcn1 has an N-terminal ubiquitin-binding domain & a unique C-terminal domain. It does not require Cys for catalysis. It interacts with the Nedd8 E2 Ubc12 on a surface that overlaps the E1-binding site. PMID: 18206966
Database Links

KEGG: sce:YLR128W

STRING: 4932.YLR128W

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