Recombinant Saccharomyces cerevisiae Epsin-1 (ENT1)

Code CSB-YP619610SVG
MSDS
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Source Yeast
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Code CSB-EP619610SVG
MSDS
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Source E.coli
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Code CSB-EP619610SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP619610SVG
MSDS
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Source Baculovirus
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Code CSB-MP619610SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ENT1
Uniprot No.
Alternative Names
ENT1; YDL161WEpsin-1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-454
Target Protein Sequence
MSKQFVRSAK NLVKGYSSTQ VLVRNATSND NHQVSKDSLI ELAEKSYDSA DFFEIMDMLD KRLNDKGKYW RHIAKALTVI DYLIRFGSEN CVLWCRENLY IIKTLKEFRH EDDEGIDQGQ IVRVKAKELT ALLSDDERLN EERNMNIKGR NRKGRRRRGT GRSDENDDDL QRAISASRLT AEEDERRRKQ DEDYETALQL SKEEEELKRL QDLQRMQQQQ GQQQLQQPMY YDIFGNPITP EEYAQFQLQQ QQQQQQQQLQ QQPMYYDVFG NPITPEELAQ FQQQQQLQEQ QYLASMQQQQ QAMSNNPFAK SEQSSSSPKR NQLVAASSPQ QLQQQKQQEP LIQNRTGNQS MTDKYSKLNE LLATGTGIDT FGNVGEARIP AQHTKTGTFI NSQGTGYRQV SDDPNHNPFL NSQYTGLPST SVVPTQTGYG FGNQSQQQSQ NNGSNNRGYT LIDL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin. Negatively regulated via phosphorylation.
Gene References into Functions
  1. X-ray analysis revealed that the crystallographic symmetry of Ent1 is primitive orthorhombic, space group P222, with unit-cell parameters a = 32.7, b = 35.5, c = 110.6 A and a diffraction limit of 2.3 A PMID: 22750874
  2. Epsin/Yap180 adaptor interaction with the scaffolds Ede1/Pan1 is required for endocytosis PMID: 18448668
Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein. Note=Localizes in a punctate pattern. Found in the actin cortical patches.
Protein Families
Epsin family
Database Links

KEGG: sce:YDL161W

STRING: 4932.YDL161W

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