Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 1A (TIF11)

Code CSB-YP007507SVG
MSDS
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Source Yeast
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Code CSB-EP007507SVG
MSDS
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Source E.coli
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Code CSB-EP007507SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007507SVG
MSDS
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Source Baculovirus
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Code CSB-MP007507SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TIF11
Uniprot No.
Alternative Names
TIF11; YMR260C; YM8156.02CEukaryotic translation initiation factor 1A; eIF-1A; Eukaryotic translation initiation factor 4C; eIF-4C
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-153
Target Protein Sequence
MGKKNTKGGK KGRRGKNDSD GPKRELIYKE EGQEYAQITK MLGNGRVEAS CFDGNKRMAH IRGKLRKKVW MGQGDIILVS LRDFQDDQCD VVHKYNLDEA RTLKNQGELP ENAKINETDN FGFESDEDVN FEFGNADEDD EEGEDEELDI DDI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.
Gene References into Functions
  1. eIF5-CTD (amino acids 241-405; Tif5p-B6) interacted with eIF1A (Tif11p). PMID: 24319994
  2. eIF1 autoregulates translation in yeast and discrimination against poor sequence context in vivo depends on specific domains and residues in eIF1, eIF1A, and eIF2beta PMID: 21930786
  3. The OB-fold is crucial for ribosome-binding and the C-terminal domain of eIF1A has eukaryotic-specific functions in ternary complex recruitment and scanning. PMID: 16193068
  4. Data suggest strongly that the interaction between eIF1A and eIF5 is involved in maintaining the fidelity of start codon AUG recognition in vivo. PMID: 16380131
  5. Location of the mutations suggests that their mechanism of suppression may depend on effects on the conserved G-protein elements: the P-loop and nucleotide-binding element. PMID: 16951075
  6. N- and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection. PMID: 17332751
  7. Our results suggest that eIF1 and eIF1A promote an open, scanning-competent preinitiation complex that closes upon start codon recognition and eIF1 release to stabilize ternary complex binding and clamp down on mRNA. PMID: 17434125
  8. A pair of repeats in the eIF1A CTT, dubbed Scanning Enhancer 1 (SE1) and SE2, was found to stimulate recruitment of Met-tRNA(i)(Met) in the ternary complex (TC) with eIF2.GTP and also to block initiation at UUG codons PMID: 20048003

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Protein Families
EIF-1A family
Database Links

KEGG: sce:YMR260C

STRING: 4932.YMR260C

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