Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 5A-2 (ANB1)

Code CSB-YP007574SVG
MSDS
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Source Yeast
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Code CSB-EP007574SVG
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Source E.coli
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Code CSB-EP007574SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007574SVG
MSDS
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Source Baculovirus
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Code CSB-MP007574SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ANB1
Uniprot No.
Alternative Names
ANB1; HYP1; TIF51B; YJR047C; J1651Eukaryotic translation initiation factor 5A-2; eIF-5A-2; Anaerobically induced protein 1; Hypusine-containing protein HP1; eIF-4D
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-157
Target Protein Sequence
SDEEHTFEN ADAGASATYP MQCSALRKNG FVVIKGRPCK IVDMSTSKTG KHGHAKVHLV TLDIFTGKKL EDLSPSTHNL EVPFVKRSEY QLLDIDDGYL SLMTMDGETK DDVKAPEGEL GDSMQAAFDE GKDLMVTIIS AMGEEAAISF KEAPRSD
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Essential for polarized growth, a process necessary for G1/S transition. May mediate large range of effects of the polyamine spermidine in the cell.
Gene References into Functions
  1. results link eIF5A function in translation with a role of signal recognition particle in the cell and may help explain the dual effects of eIF5A in differential and general translation PMID: 24306454
  2. Eukaryotic translation initiation factor (eIF) 5A stimulates protein synthesis in Saccharomyces cerevisiae. PMID: 21451136
Subcellular Location
Cytoplasm. Note=Concentrates in the perinuclear region.
Protein Families
EIF-5A family
Database Links

KEGG: sce:YJR047C

STRING: 4932.YJR047C

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