Product Details

Purity

>85% (SDS-PAGE)

Target Names

SPT16

Uniprot No.

P32558

Alternative Names

SPT16; CDC68; SSF1; YGL207W; FACT complex subunit SPT16; Cell division control protein 68; Facilitates chromatin transcription complex subunit SPT16; Suppressor of Ty protein 16

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Transcription elongation is promoted by the repression of transcription initiation from cryptic sites. Also acts in establishing transcription initiation complexes and promotes SPT15/TBP-binding to a TATA box. Together with replication factor-A protein (RPA), FACT may play a role in nucleosome deposition during DNA replication.

Gene References into Functions

SPT16 interactions with nucleosome. PMID: 29271283
Results show that Spt16 depletion results in global changes in nucleosome fuzziness and position shifts and that the majority of these changes are suppressed by inhibiting RNA polymerase II supporting a model in which Spt16 has a role in maintaining local nucleosome stability. PMID: 27141053
These results suggest that the large scale overlap in Mot1 and Spt16 function arises from a combination of both their unique and shared functions in transcription complex assembly and chromatin structure regulation. PMID: 27226635
Spt6 and FACT have both overlapping and distinct functional interactions with histones and nucleosomes and that binding to each of the components of a nucleosome is not sufficient to produce nucleosome reorganization PMID: 26416482
results suggest that the discrimination of Spt16 to Htz1-bound genes is due to the priority of Chz1 over Spt16 in binding to the Htz1-bound genomic regions; Chz1-escorted Htz1 therefore impairs Spt16 binding at chromatin PMID: 24707933
The Spt16 subunit of FACT is specifically required for H2B deposition onto rDNA genes. PMID: 23689130
Structure of the Spt16 middle domain reveals functional features of the histone chaperone FACT. PMID: 23417676
Mutation that encodes the Spt16 subunit of FACT causes phenotypes associated with defects in transcription and replication. PMID: 21625001
These findings identify Spt16 as an Rtt101 substrate, and suggest that Spt16 ubiquitylation is important for FACT to function during DNA replication. PMID: 20634314
The FACT subunit Spt16 and Pkc1 signaling have an overlapping essential function, with an unexpected role for FACT in the maintenance of cell integrity. PMID: 19727824
N-terminal region of Polalpha (Pol1p) that is evolutionarily conserved among different species interacts with Spt16p-Pob3p and Ctf4p in vivo PMID: 15485923
The Spt16 N-terminal domain does become important under conditions of replication stress, where it mediates adaptation to stress conditions. PMID: 15520471
Chd1 and SPT16 and POB3 act in opposition in regulating transcription. PMID: 17620414
neither the Spt16 NTD nor a key residue in the putative Pob3-M-binding site was required for interactions with histone N termini or for yFACT-mediated nucleosome reorganization PMID: 18089575
Spt16 restores normal nucleosome structure by redepositing the displaced H3-H4 histones, thereby preventing incorporation of new histones. PMID: 19683500

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Subcellular Location

Nucleus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.

Protein Families

Peptidase M24 family, SPT16 subfamily

Database Links

KEGG: sce:YGL207W

STRING: 4932.YGL207W

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Recombinant Saccharomyces cerevisiae FACT complex subunit SPT16 (SPT16), partial

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