Product Details

Uniprot No.

Target Names

SPT16

Alternative Names

FACT complex subunit SPT16 (Cell division control protein 68) (Facilitates chromatin transcription complex subunit SPT16) (Suppressor of Ty protein 16) SPT16 CDC68 SSF1 YGL207W

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SPT16 protein (647-964aa)

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The SPT16 polyclonal antibody is produced by immunizing a rabbit with a recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SPT16 protein (647-964aa). The rabbit will mount a humoral response to the immunogen and the antibodies produced can be harvested by bleeding the rabbit to obtain IgG-rich serum. The resulting SPT16 antibody is subjected to affinity chromatography purification. It shows reactivity with Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SPT16 protein. The functionality of this SPT16 antibody has been validated in ELISA and WB applications.

SPT16 is a component of the FACT complex, which is involved in the reorganization of nucleosomes during transcription. This protein plays a crucial role in maintaining proper chromatin structure, facilitating transcription initiation and elongation, and ensuring accurate gene expression. SPT16 helps prevent the stalling of RNA polymerase and aids in the smooth progression of the transcription process, ultimately contributing to gene regulation and efficient transcription in yeast cells.

Target Background

Function

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Transcription elongation is promoted by the repression of transcription initiation from cryptic sites. Also acts in establishing transcription initiation complexes and promotes SPT15/TBP-binding to a TATA box. Together with replication factor-A protein (RPA), FACT may play a role in nucleosome deposition during DNA replication.

Gene References into Functions

SPT16 interactions with nucleosome. PMID: 29271283
Results show that Spt16 depletion results in global changes in nucleosome fuzziness and position shifts and that the majority of these changes are suppressed by inhibiting RNA polymerase II supporting a model in which Spt16 has a role in maintaining local nucleosome stability. PMID: 27141053
These results suggest that the large scale overlap in Mot1 and Spt16 function arises from a combination of both their unique and shared functions in transcription complex assembly and chromatin structure regulation. PMID: 27226635
Spt6 and FACT have both overlapping and distinct functional interactions with histones and nucleosomes and that binding to each of the components of a nucleosome is not sufficient to produce nucleosome reorganization PMID: 26416482
results suggest that the discrimination of Spt16 to Htz1-bound genes is due to the priority of Chz1 over Spt16 in binding to the Htz1-bound genomic regions; Chz1-escorted Htz1 therefore impairs Spt16 binding at chromatin PMID: 24707933
The Spt16 subunit of FACT is specifically required for H2B deposition onto rDNA genes. PMID: 23689130
Structure of the Spt16 middle domain reveals functional features of the histone chaperone FACT. PMID: 23417676
Mutation that encodes the Spt16 subunit of FACT causes phenotypes associated with defects in transcription and replication. PMID: 21625001
These findings identify Spt16 as an Rtt101 substrate, and suggest that Spt16 ubiquitylation is important for FACT to function during DNA replication. PMID: 20634314
The FACT subunit Spt16 and Pkc1 signaling have an overlapping essential function, with an unexpected role for FACT in the maintenance of cell integrity. PMID: 19727824
N-terminal region of Polalpha (Pol1p) that is evolutionarily conserved among different species interacts with Spt16p-Pob3p and Ctf4p in vivo PMID: 15485923
The Spt16 N-terminal domain does become important under conditions of replication stress, where it mediates adaptation to stress conditions. PMID: 15520471
Chd1 and SPT16 and POB3 act in opposition in regulating transcription. PMID: 17620414
neither the Spt16 NTD nor a key residue in the putative Pob3-M-binding site was required for interactions with histone N termini or for yFACT-mediated nucleosome reorganization PMID: 18089575
Spt16 restores normal nucleosome structure by redepositing the displaced H3-H4 histones, thereby preventing incorporation of new histones. PMID: 19683500

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Subcellular Location

Nucleus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.

Protein Families

Peptidase M24 family, SPT16 subfamily

Database Links

KEGG: sce:YGL207W

STRING: 4932.YGL207W

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Code	CSB-PA022955XA01SVG
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: SPT16 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65 kDa Observed band size: 65 kDa
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SPT16 Antibody

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