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Recombinant Saccharomyces cerevisiae G1/S-specific cyclin CLN2 (CLN2), partial

Recombinant Saccharomyces cerevisiae G1/S-specific cyclin CLN2 (CLN2), partial

Code	CSB-YP324042SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP324042SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP324042SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP324042SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP324042SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

CLN2

Uniprot No.

P20438

Alternative Names

CLN2; YPL256CG1/S-specific cyclin CLN2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Essential for the control of the cell cycle at the G1/S (start) transition. Interacts with the CDC28 protein kinase to form MPF.

Gene References into Functions

A docking interface in the cyclin Cln2 promotes multi-site phosphorylation of substrates and timely cell-cycle entry. PMID: 25619768
We carried out a focused study on a physiologically important nucleosome-depleted regions in the yeast CLN2 promoter. Eight conserved factor binding sites, including that of Reb1, Mcm1, and Rsc3, that cause the local nucleosome depletion, were identified. PMID: 21596311
Reporter assays show that both activities appear to depend on the 5'-untranslated region of CLN2 mRNA and that Ssd1p binds to this region via its amino- and carboxy-terminal domains. PMID: 20977549
analysis of NDR- or nucleosomal-localized SCB sites driving Cln2 expression PMID: 20412770
positive-feedback-amplified expression of Cln1 and Cln2 simultaneously drives robust budding and rapid, coherent regulon PMID: 18633409
Forced periodic expression of the G(1) cyclin CLN2 phase locks the cell cycle of budding yeast over a range of extrinsic periods in an exponentially growing monolayer culture. PMID: 19346485