Recombinant Saccharomyces cerevisiae GTPase-activating protein GYP6 (GYP6)

Code CSB-YP329201SVG
MSDS
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Source Yeast
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Code CSB-EP329201SVG
MSDS
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Source E.coli
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Code CSB-EP329201SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP329201SVG
MSDS
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Source Baculovirus
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Code CSB-MP329201SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GYP6
Uniprot No.
Alternative Names
GYP6; YJL044C; J1202; GTPase-activating protein GYP6; GAP for YPT6
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-458
Target Protein Sequence
MKDVLQWAID HYETRDQLVQ KGIWKGELYH DSIFKENSRG WLWKALLLCD ENNNCLLTDN FKGLDLNQFG LVPVPMLADG DNYDENHNAN VPKRVLHSNV SSSVGIRRLT PVEAVEKHPL SDDNDKTKGS LSKGSDEKPL TLRETLEIID LDLSRIMLDD IFQEPKVHAQ MRQLLYNYLL IHQSEHLQYK QGFHEILSVI YLQLYHGTDL DNTDLQNVLI IFNKLMNQIE PIFYNEENLI NWDKRVFTKI FRICLPDLFS KVFYQPPKTG SGKKKNVDHL IHSNLIWLIR WTRLLFLREL PLKYVLIVWD HVLTFNYPLD IFIACTIITL LLSIYDELHE LVSQGDYEHT NNNDEFVELI LHFKKIFEKE DASKDDEKFL DLCKVTGNLC ELWYGKNYDD MRLICDTFIN AKFGIKTSDV LSMETAKLTI DPNRQSLENK LRERVRQTIL KNKKKISQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Stimulates specifically the GTPase activity of YPT6.
Gene References into Functions
  1. Gyp6, a putative GAP for Ypt6, binds to Ypt32 and is recruited to the membrane, facilitating the dissociation of Ypt6. PMID: 24194547
Subcellular Location
Cytoplasm.
Database Links

KEGG: sce:YJL044C

STRING: 4932.YJL044C

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