Recombinant Saccharomyces cerevisiae General control protein GCN4 (GCN4)

1. The down-regulation of the GCN4 expression (Gcn(-) phenotype) in the eIF5(G31R) mutant was not because of leaky scanning defects; rather was due to the utilization of upUUG initiation codons at the 5' regulatory region present between uORF1 and the main GCN4 ORF. PMID: 28385532
2. GCN4 is required for nitrogen catabolite repression-sensitive nuclear Gln3 localization. PMID: 28007891
3. Gcn4 sumoylation in limiting its association with chromatin. PMID: 27770033
4. Presented are molecular dynamics simulations reflecting a secondary structure of phopspho-GCN4 at different pH. PMID: 28230348
5. Reinitiation on GCN4 mRNA after translation of its four short uORFs is regulated by the sequence immediately following their respective stop codons. PMID: 26822200
6. Upstream-open reading frame 2 (uORF2) from the GCN4 mRNA leader resembles the well-established reinitiation (REI)-permissive uORF1 in its high REI-promoting activity. PMID: 24623812
7. our results suggest that the folding behavior of the well-studied GCN4 leucine-zipper domain is more complex than was previously appreciated. PMID: 23116373
8. Elucidated RNA binding interface of the GCN4 leucine zipper motif from yeast. Site-directed mutagenesis of the LZ-GCN4 RNA binding interface showed that substrate binding is facilitated by lysine and arginine side chains. PMID: 22374868
9. the combination of Gln3 and Gcn4 results in the constitution of a hybrid modulator which elicits a novel transcriptional response, not evoked when these modulators act in a non-combinatorial fashion. PMID: 21184740
10. Gcn4(L267S) variant carries an amino acid substitution in the third of four conserved leucines of the zipper dimerization domain. This abolished FLO11 expression and resulted in reduced transcriptional activity for amino acid biosynthetic genes. PMID: 21111745
11. GAAC is a major effector of the TOR pathway, with Gcn4p and Gln3p each inducing a similar number of genes during rapamycin treatment PMID: 20233714
12. interactions between the two nanorope-like assemblies are due to the hydrophobic residues on the leucine faces, which are also responsible for the dimer formation of the wild-type GCN4 leucine zipper. PMID: 20027625
13. eIF3b functions between 48S assembly and subunit joining to influence AUG recognition and reinitiation on GCN4 mRNA PMID: 14976554
14. Transcriptional activator Gcn4p recruits SAGA and Srb mediator to the upstream activation sequence of the promoter regions. PMID: 15831453
15. Site-specific photocrosslinkers positioned within the central transcription-activating region of yeast Gcn4 were used to identify three polypeptides in direct physical proximity to the activator during the process of transcription activation. PMID: 15866178
16. eIF5 is critical for the integrity of the scanning preinitiation complex and the accurate control of GCN4 translation. PMID: 15964804
17. Simultaneous recruitment of trans-activators by GCN4 stimulates multiple steps of transcription in vivo. PMID: 15964818
18. GCN4 expression is induced by sodium and has a role in controlling the accumulation of the 5' to 3' RNA degradation inhibitor, 3'-phosphoadenosine 5'-phosphate PMID: 16352596
19. large fraction of genes activated by Gcn4p are repressed by H3 and H4 amino termini under non-inducing conditions, indicating that one function of Gcn4p is to overcome chromatin-mediated repression that depends on histone tails PMID: 16461773
20. report here the design of a modified GCN4-bZIP DNA-binding protein with an azobenzene chromophore introduced between Cys residues at positions 262 and 269 (S262C, N269C) within the zipper domain PMID: 16681380
21. a set of native or nativelike tertiary interactions, distributed throughout the entire sequence, collectively stabilize the folding transition state of the GCN4 coiled coil. PMID: 16953584
22. Results describe the CPC1 regulon, the ortholog of Saccharomyces cerevisiae GCN4, by profiling transcription in wild-type and cpc-1 mutant strains. PMID: 17449655
23. LZGCN4 x-form is generally less structured in comparison with the classical GCN4-p1 coiled coil, but still retains a structured alpha-helical central core PMID: 17469817
24. Rpn13 protein and Rpn14 protein are involved in the efficient recognition of 26S proteasome for the proteolysis of ubiquitinated Gcn4 protein. PMID: 17499717
25. Gcn4 is required for the response to peroxide stress in the yeast Saccharomyces cerevisiae PMID: 18417611
26. Genetic epistasis analyses suggest that dietary restriction, reduced 60S subunit abundance, and Gcn4 activation extend yeast life span by similar mechanisms. PMID: 18423200
27. Results suggest that decreasing cargo proteins in the late endosome through impaired delivery or enhanced degradation is required to attenuate substantially transcriptional activation by Gcn4. PMID: 18794364
28. Adaptation to methylglyoxal was impaired in gcn4Delta cells, indicating the expression of certain genes regulated by Gcn4 to be important for the adaptive response to methylglyoxal. PMID: 18812164
29. 5,764 mRNAs, 54 metabolites, and 83 experimental (13)C-based reaction fluxes were quantified in continuous cultures of yeast under stress in the absence or presence of global regulator Gcn4p. PMID: 19346491

Show More

Hide All

KEGG: sce:YEL009C

STRING: 4932.YEL009C