Recombinant Saccharomyces cerevisiae Guanine nucleotide exchange factor SRM1 (SRM1)

Code CSB-YP324267SVG
MSDS
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Source Yeast
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Code CSB-EP324267SVG
MSDS
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Source E.coli
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Code CSB-EP324267SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP324267SVG
MSDS
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Source Baculovirus
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Code CSB-MP324267SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SRM1
Uniprot No.
Alternative Names
SRM1; MTR1; PRP20; YGL097W; Guanine nucleotide exchange factor SRM1; Pheromone response pathway component SRM1; Pre-mRNA-processing protein 20; Regulator of chromosome condensation; Suppressor of receptor mutations 1; mRNA transport protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-482
Target Protein Sequence
MVKRTVATNG DASGAHRAKK MSKTHASHII NAQEDYKHMY LSVQPLDIFC WGTGSMCELG LGPLAKNKEV KRPRLNPFLP RDEAKIISFA VGGMHTLALD EESNVWSWGC NDVGALGRDT SNAKEQLKDM DADDSSDDED GDLNELESTP AKIPRESFPP LAEGHKVVQL AATDNMSCAL FSNGEVYAWG TFRCNEGILG FYQDKIKIQK TPWKVPTFSK YNIVQLAPGK DHILFLDEEG MVFAWGNGQQ NQLGRKVMER FRLKTLDPRP FGLRHVKYIA SGENHCFALT KDNKLVSWGL NQFGQCGVSE DVEDGALVTK PKRLALPDNV VIRSIAAGEH HSLILSQDGD LYSCGRLDMF EVGIPKDNLP EYTYKDVHGK ARAVPLPTKL NNVPKFKSVA AGSHHSVAVA QNGIAYSWGF GETYAVGLGP FEDDTEVPTR IKNTATQDHN IILVGCGGQF SVSGGVKLSD EDAEKRADEM DD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Guanine nucleotide exchange factor that promotes the exchange of GSP1/GSP2-bound GDP by GTP and controls RNA metabolism and transport. Involved in yeast pheromone response pathway and in mRNA metabolism. Involved in nuclear pore complex (NPC) assembly and required for mRNA and ribosome nuclear export. Binds chromatin and is involved NPC-mediated transcriptional control.
Gene References into Functions
  1. Study reveals that Prp20p and RCC1 share similar Ran GTPase binding mode. PMID: 21093592
Subcellular Location
Nucleus.
Database Links

KEGG: sce:YGL097W

STRING: 4932.YGL097W

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