Recombinant Saccharomyces cerevisiae High-affinity hexose transporter HXT6 (HXT7), partial

Code CSB-YP340043SVG
MSDS
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Source Yeast
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Code CSB-EP340043SVG
MSDS
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Source E.coli
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Code CSB-EP340043SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP340043SVG
MSDS
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Source Baculovirus
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Code CSB-MP340043SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HXT7
Uniprot No.
Alternative Names
HXT7; YDR342C; D9651.11; High-affinity hexose transporter HXT7
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
High-affinity glucose transporter.
Gene References into Functions
  1. turnover of both Hxt3 and Hxt7 is dependent on the essential E3 ubiquitin ligase, Rsp5, indicating that the role of the Vid30c might be indirect of Hxt ubiquitylation PMID: 23227176
  2. The amino acid side chain length in transmembrane segment 5 of HXT7 is important for glucose affinity. PMID: 21892826
  3. Variation in the copy number of HXT6/HTX7 locus was observed in a survey of ~70 yeast strains. PMID: 21552329
  4. Saccharomyces cerevisiae was engineered for enhanced arsenite accumulation by overexpression of transporters responsible for the influx of the contaminant. The transporter genes FPS1 and HXT7 were cloned under the control of the late-phase ADH2-promoter. PMID: 20638426
  5. Asp(340) is situated at or close to a substrate recognition site and is a key residue determining high-affinity glucose transport by Hxt7 PMID: 20525688
  6. Saccharomyces galactose transporter system could be a highly effective xylose transporter. The information reported here should be of great importance for improving the Saccharomyces yeast transport of xylose. PMID: 15197732
  7. promoter produces higher invertase activity at 139- and 30-fold of the basal activity in strains GN 3C.2 and W303-1, respectively PMID: 17520177

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Major facilitator superfamily, Sugar transporter (TC 2.A.1.1) family
Database Links

KEGG: sce:YDR342C

STRING: 4932.YDR342C

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