Recombinant Saccharomyces cerevisiae Histidine protein methyltransferase 1 (HPM1)

Code CSB-YP334722SVG
MSDS
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Source Yeast
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Code CSB-EP334722SVG
MSDS
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Source E.coli
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Code CSB-EP334722SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP334722SVG
MSDS
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Source Baculovirus
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Code CSB-MP334722SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HPM1
Uniprot No.
Alternative Names
HPM1; MNI1; YIL110W; Histidine protein methyltransferase 1; EC 2.1.1.85; Mitotic exit network interactor 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-377
Target Protein Sequence
MSFSFGFTSN DFDDDELVAQ PETFVESSKE NENTTAYINP LDSDFLSQAG VVQPNVEDLG TILESLKDVR LTFEEFQSPI YRKPLIKREL FDVKHQLMLE TDAQSNNNST ELDILLGDTS EDLRKNIYEG GLKSWECSYD LVDLLSENVD RISNDIDAVV EIGCGTALPS EFLFRSALLR NDRSKGLKFV LTDYNASVLR LVTIPNLVIT WAKTVLTKEQ WYALQKDECE DIPINNEELL LTSKLLAAFY DDVQSRNISV TLISGSWGRK FSNLIHEVLS GSQKVLSLSS ETIYQPDNLP VIAETILDIH NLPQTDVKTY VAAKDIYFGV GGSITEFEAY LDDKINSEHL PIHSERFKVN SGLKRSIICI ETNKAIR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein-histidine N-methyltransferase that mediates methylation of RPL3 at 'His-243'. Methylates ribosome-associated RPL3, but not free RPL3, thereby regulating 60S subunit assembly. In addition to RPL3, mediates His methylation of other proteins.
Gene References into Functions
  1. Hpm1 is a multifunctional methyltransferase with independent roles in ribosome biogenesis and translation. PMID: 26826131
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Methyltransferase superfamily, METTL18 family
Database Links

KEGG: sce:YIL110W

STRING: 4932.YIL110W

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