Recombinant Saccharomyces cerevisiae Histone H3-like centromeric protein CSE4 (CSE4)

Code CSB-YP339622SVG
MSDS
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Source Yeast
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Code CSB-EP339622SVG
MSDS
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Source E.coli
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Code CSB-EP339622SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339622SVG
MSDS
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Source Baculovirus
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Code CSB-MP339622SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CSE4
Uniprot No.
Alternative Names
CSE4; CSL2; YKL049C; YKL262; Histone H3-like centromeric protein CSE4; CENP-A homolog; Chromosome segregation protein 4
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-229
Target Protein Sequence
MSSKQQWVSS AIQSDSSGRS LSNVNRLAGD QQSINDRALS LLQRTRATKN LFPRREERRR YESSKSDLDI ETDYEDQAGN LEIETENEEE AEMETEVPAP VRTHSYALDR YVRQKRREKQ RKQSLKRVEK KYTPSELALY EIRKYQRSTD LLISKIPFAR LVKEVTDEFT TKDQDLRWQS MAIMALQEAS EAYLVGLLEH TNLLALHAKR ITIMKKDMQL ARRIRGQFI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for functional chromatin architecture at the yeast 2-micron circle partitioning locus and promotes equal plasmid segregation.
Gene References into Functions
  1. findings suggest that a stable Cse4 nucleosome is maintained by dynamic chaperone-in-residence Sc PMID: 24844245
  2. The budding yeast centromere DNA element II wraps a stable Cse4 hemisome in either orientation in vivo. PMID: 24737863
  3. Cse4 localization at a functional kinetochore does not require the recognition of a specific DNA sequence by the CBF3 complex; rather, its localization depends on stable interactions among kinetochore proteins. PMID: 25533342
  4. Report show that Fpr3 regulates Cse4 proteolysis and the structural changes in CSe4 might be important for its interaction with Psh1. PMID: 24514906
  5. phosphorylation is crucial in Psh1-assisted control of Cse4 levels and the Psh1-Cse4 association itself functions to prevent Cse4 misincorporation PMID: 25183013
  6. Results indicate that Cse4 phosphorylation and its role in promoting faithful chromosome segregation. PMID: 23637466
  7. N terminus of Cse4 is a target of ubiquitination and that this modification plays a major role in regulating its proteolytic degradation. PMID: 23525333
  8. The budding yeast Saccharomyces cerevisiae centromere recruits two Cse4 (ScCENP-A) molecules, which are deposited during S phase and remain stably bound through late anaphase. The authors suggest that the centromere incorporates a Cse4-H4 tetramer. PMID: 23623551
  9. Our results favor a H3-Cse4 heterotypic octamer at the budding yeast centromere PMID: 22693454
  10. Methylation of CenH3 (Cse4) arginine 37 regulates kinetochore integrity and chromosome segregation PMID: 22615363
  11. the occupancy of Cse4 at STB induces positive DNA supercoiling, with a linking difference (DeltaLk) contribution estimated between +1 and +2 units PMID: 21807992
  12. These data suggest a role for Snf2 in the maintenance of point centromeres involving the removal of Cse4 from ectopic sites. PMID: 21505420
  13. The association of Cse4 with the STB (zinc cluster DNA-binding proteins) is dependent on Scm3, the loading factor responsible for the incorporation of Cse4 into the nucleosome. PMID: 21173161
  14. Data show that that the inner kinetochore complex (CBF3) is required for pericentric DNA looping at the Cse4p-containing nucleosome. PMID: 19656849
  15. We demonstrate that Cse4 resides at the partitioning locus STB of the 2-microm plasmid. Cse4p-STB association is absolutely dependent on the plasmid partitioning proteins Rep1p and Rep2p and the integrity of the mitotic spindle. PMID: 16966420
  16. Experimental evidence supports the model that the Cse4 nucleosome is an octamer, containing two copies each of Cse4, H2A, H2B, and H4. PMID: 19782029

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Subcellular Location
Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres.
Protein Families
Histone H3 family
Database Links

KEGG: sce:YKL049C

STRING: 4932.YKL049C

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