Recombinant Saccharomyces cerevisiae Histone chaperone RTT106 (RTT106)

Code CSB-YP328081SVG
MSDS
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Source Yeast
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Code CSB-EP328081SVG
MSDS
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Source E.coli
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Code CSB-EP328081SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP328081SVG
MSDS
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Source Baculovirus
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Code CSB-MP328081SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RTT106
Uniprot No.
Alternative Names
Histone chaperone rtt106; Regulator of Ty1 transposition protein 106; RT106_YEAST; rtt106
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-455
Target Protein Sequence
SKLFLDELP ESLSRKIGTV VRVLPSSLEI FEELYKYALN ENSNDRSGRH KKPRIDVSSD LLKTDEISET NTIFKLEGVS VLSPLRKKLD LVFYLSNVDG SPVITLLKGN DRELSIYQLN KNIKMASFLP VPEKPNLIYL FMTYTSCEDN KFSEPVVMTL NKENTLNQFK NLGLLDSNVT DFEKCVEYIR KQAILTGFKI SNPFVNSTLV DTDAEKINSF HLQCHRGTKE GTLYFLPDHI IFGFKKPILL FDASDIESIT YSSITRLTFN ASLVTKDGEK YEFSMIDQTE YAKIDDYVKR KQMKDKSMSE ELKAKSKSKG QATDGTADQP SILQEATRQM QDEKKAGVFS DDDEENDQNF EAESDLSDGS GQESSDGAED GEEAEEDDEE DDEEEDKKGQ SALNRDNSFA SINGQPEQEL QYKEFKEPLE LEDIPIEIDN DDDEDDEDGS GVEYD
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Histones H3 and H4 chaperone involved in the nucleosome formation and heterochromatin silencing. Required for the deposition of H3K56ac-carrying H3-H4 complex onto newly-replicated DNA. Plays a role in the transcriptional regulation of the cell-cycle dependent histone genes by directly recruiting the SWI/SNF and RSC chromatin remodeling complexes to the histone genes in a cell cycle dependent manner. In cooperation with HIR and ASF1, creates a repressive structure at the core histone gene promoter and contributes to their repression outside of S phase. Involved in regulation of Ty1 transposition.
Gene References into Functions
  1. results suggested a unique regulatory mechanism in which Rtt106 sensed the level of histone proteins to maintain the proper level of histone gene transcription PMID: 22907759
  2. tight connection between DNA synthesis and histone deposition during DNA replication mediated by H3K56ac/CAF1/Rtt106 provides a mechanism for the stabilization of advancing replication forks and the maintenance of genome integrity. PMID: 22102830
  3. Results uncover the structural basis of the diverse functions of Rtt106p and provide new insights into its cellular roles. PMID: 20007951
  4. There is a new role of CAF-1 and Rtt106p in epigenetic silencing and indicate that the spreading of heterochromatin, a poorly understood process, requires histone chaperones. PMID: 17410207
  5. a direct link for Rtt106 with transcription elongation and the chromatin dynamics associated with RNA polymerase II passage. PMID: 18708354

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Subcellular Location
Nucleus. Chromosome.
Protein Families
RTT106 family
Database Links

KEGG: sce:YNL206C

STRING: 4932.YNL206C

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