Product Details

Purity

>85% (SDS-PAGE)

Target Names

ISU1

Uniprot No.

Q03020

Alternative Names

ISU1; NUA1; YPL135W; Iron sulfur cluster assembly protein 1; mitochondrial; Iron sulfur cluster scaffold protein 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Expression Region

28-165

Target Protein Sequence

SSI TKRLYHPKVI EHYTHPRNVG SLDKKLPNVG TGLVGAPACG DVMRLQIKVN DSTGVIEDVK FKTFGCGSAI ASSSYMTELV QGMTLDDAAK IKNTEIAKEL SLPPVKLHCS MLAEDAIKAA IKDYKSKRNT PTMLS

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold proteins ISU1 and ISU2. In a second step, the cluster is released from ISU1/ISU2, transferred to glutaredoxin GRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISU1/ISU2 depends on the function of the cysteine desulfurase complex NFS1-ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin (YFH1) as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase ARH1 and ferredoxin YAH1, which receive their electrons from NADH. Fe-S cluster release from ISU1/ISU2 is achieved by interaction with the Hsp70 chaperone SSQ1, assisted by the DnaJ-like co-chaperone JAC1 and the nucleotide exchange factor MGE1. ISU1 is the major isoform in yeast, while ISU2 is not detectable in cells grown to stationary phase. Also involved in production of a sulfur precursor required for thiolation of cytoplasmic tRNAs.

Gene References into Functions

Isu is a substrate of the Lon-type protease and that it is protected from degradation by Nfs1, the sulfur donor for Fe-S cluster assembly, as well as by Jac1, the J-protein Hsp70 cochaperone that functions in cluster transfer from Isu. Nfs1 and Jac1 variants known to be defective in interaction with Isu were also defective in protecting Isu from degradation. PMID: 26842892
The eukaryotic forms are critical for Fe-S cluster assembly whereas prokaryotic forms are more dispensable. We found that a key to this difference is a single amino acid in the scaffold protein Isu1 at position 141. PMID: 25996596
Fe/S cluster synthesis on Isu1 requires the ferredoxin Yah1 PMID: 25358379
In frataxin-deleted strains expressing the bypass mutant Isu1, cell growth and iron homeostasis are restored to close to normal. PMID: 24433162
The present paper reported that a single amino acid substitution in the mature form of Isu1 restored many deficient functions in yfh1 or frataxin-depleted yeast cells. PMID: 21936771
The studies indicate that the Isu proteins and the iron-sulfur pathway can donate iron to Sod2p. PMID: 19561359

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Subcellular Location

Mitochondrion matrix.

Protein Families

NifU family

Database Links

KEGG: sce:YPL135W

STRING: 4932.YPL135W

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Recombinant Saccharomyces cerevisiae Iron sulfur cluster assembly protein 1, mitochondrial (ISU1)

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